About 90% of Cynorkis species are endemic to the biodiversity hotspot Madagascar. This terrestrial habitat-specific genus received little study for fungal diversity support conservation. We evaluated culturable fungi 11 and soil characteristics from six sites spanning a >40 km radius in along region’s inselbergs. Peloton-forming were grown vitro root/protocorm slices positively identified us...

The present study describes the encapsulation of protocorm of Cymbidium bicolor Lindl. from 60 days-old in seed cultures for short- term conservation and propagation. Various concentrations and combinations of gelling matrix (sodium alginate) and complexing agents (calcium chloride) were tested to prepare uniform beads. The ideal beads were ob- tained through a combination of 3% sodium alginate...

Carbon and nitrogen are the two elements that most affect plant organogenesis. In vitro, usually as part of the macronutrients. Some anecdotal evidence from the literature suggests that the ammonium (NH4+) to nitrate (NO3–) ratio may affect orchid organogenesis. In this study, to test this hypothesis, different NH4+: NO3– ratios were tested on the development of protocorm-like bodies (PLBs) of ...

In Indonesia, the agribusiness sector has begun to expand due increase in entrepreneurship of orchid plants. Several genera and species plants are promising be traded as ornamental plants, one which is moon (Phalaenopsis amabilis). However, limited number seeds or produced by conventional propagation methods takes a long time get new tillers. Therefore, tissue culture techniques can used grow r...