background the development of an effective subunit vaccine against brucellosis is a research area of intense. but optimization of recombinant proteins production in escherichia coli and content of endotoxins associated with final recombinant proteins are very important. objectives in the present study, expression and purification of brucella melitensis rhsp and rtf were optimized to reduce endo...

Introduction: Vaccine studies against Pseudomonas aeruginosa have often focused on outer membrane proteins (OPRs) due to their potent stimulation of the immune response. Using major outer membrane proteins of cell walls (mOMPs) of P. aeruginosa and other Gram-negative bacteria actively stimulate the immune system without any toxic side effects. Moreover, these antigens show immunological cross-...

Plants are often subjected to several environmental stresses throughout their life cycle. Adaptation of plants to saline conditions may be due to some salt-related changes in the pattern of gene(s) expression. The physiological role of stress induced proteins is not yet clearly understood. However, it is believed that these proteins allow plants to produce biochemical and structural adjustments...

secale montanum is one of the important perennial grasses growingnaturally in arid to semiarid pastures and rangelands, with a typical mediterraneanclimate, in northern and western iran at altitudes of 800-2900 m. in this paper, seedstorage protein profiles of nine wild populations of s. montanum from differentregions of iran and their phenotypically superior progenies as well as a multi-origin...

analyzing the synovial fluid is one of the common methods to diagnose the articular problems to detect the stage and express the prognosis. such gross, cytological, and biochemical analysis of synovial fluids can aid in the diagnosis of various joint diseases. objectives: normal values for synovial fluid in the camels have been described previously; however, there are no reports regarding conce...

abstract deep seated cavernous angioma (ca) is a very rare entity, those occurring in the hypothalamus are still less common. we present a case of a 52-year-old man who presented with behavior and memory disturbance attacks. he had a ca of the hypothalamus as revealed by magnetic resonance imaging (mri). we will discuss the role and importance of imaging in such scenario and also the differenti...

neurofibroma is an autosomal dominant disorder which has major criteria such as hyperpigmentation (cafe-au lait spots), cutaneous and subcutaneous tumors and bone deformities. in this report, a case of multifocal intraosseous neurofibroma in a 16-year-old male with right facial asymmetry, multiple unerupted maxillary posterior teeth and a previous history of infratemporal and orbital neurofibro...

background: trastuzumab (herceptin) is a humanized monoclonal antibody (mab) which is used for specific treatment of metastatic breast cancer in patients with overexpression of her2/neu receptor. in this study, we have attempted to develop a biosimilar version of trastuzumab mab. methods: according to in silico studies, the heavy and light chains of trastuzumab mab were designed and constructed...

expressions of recombinant proteins for different applications are important objectives in molecular biotechnology; however, expression of some recombinant proteins is difficult. several methods have been designed for expression of these proteins. the aim of this study was to construct a vector containing mtb32c fragment of mycobacterium tuberculosis (m.tuberculosis) as a fusion partner in orde...