The clinical diagnosis of definite multiple sclerosis is supported by abnormalities in the cerebrospinal fluid: variable mild pleocytosis and elevation of total protein, moderately elevated total IgG in most patients, and the almost invariable presence of discrete immunoglobulins after electrophoresis, the oligoclonal bands. The oligoclonal bands are non-specific, and are seen in most diseases ...

Background: Male infertility has many causes, including genetic infertility. The NOP2/Sun domain family, member7 (Nsun7) gene, which encodes putative methyltransferase Nsun7, has a role in sperm motility. The aim of the present study was to investigate the effect of the T26248G polymorphism on Nsun7 protein function and its role in male infertility. Materials and Methods: Semen samples were col...

Patients who are allergic to latex (Hevea brasiliensis) may exhibit cross-hypersensitivity with foods. We present a case of anaphylaxis due to cassava in a patient suffering from pollinosis, latex allergy, and latex-fruit syndrome. We performed sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting with cassava, avocado, chestnut, banana, kiwi, and latex extract...

NADPH oxidase is an enzyme in the plasma membrane of the neutrophil that catalyzes the production of 02 #{149}a species central to the oxygen-dependent killing mechanisms of this cell. The oxidase is dormant in resting cells and is activated upon the addition of a stimulus. Neutrophils of patients with chronic granulomatous disease (CGD) manifest no oxidase activity when stimulated. The possibl...

Seed protein quality is an important topic in the production of soybean. The quality of soybean proteins is limited by anti-nutrient proteins and low levels of essential sulfur amino acids. In this study, protein content and solubility of six cultivars were evaluated and seed storage proteins were analyzed using SDS-PAGE and scanning densitometry. The results showed that seed storage protein ba...

The taxonomic affinity of six species of Crocus (C, cancellatus subsp. Damascenus C. almehensis, C. gilanicus, C. sativus, C.pallassi subsp. haussknechtii,C. speciosus subsp. speciosus) from Iran were studied usingdifferent electrophoresis techniques. The similarity index of the species was computed by comparison of protein bands of - Corm and seed tissues, and the related dendrogms of affi...

We will first prepare reference antisera to total proteins of P. cinnamomi and P. citricola. The protein profile of each fungus will be analyzed by SDS-Polyacrylamide Gel Electrophoresis(PAGE). By this procedure, fungal proteins are driven by an electric current and are separated into bands in a gel medium. The distance of the movement of each protein is dictated by its size. The protein bands ...

Effect of various NaCl treatments (0, 50, 100, 200, and 300 mM) at different growth and development stages (tillering, boot swellen, flowering and anthesis) in two wheat cultivars (Ghods: salt – sensitive, Boolani: salt – resistant) on SDS-PAGE electrophoretic pattern of leaf proteins was studied under greenhouse conditions. Generally, in response to salinity treatments, the decrease in protein...

The U(L)3 open reading frame (ORF) has the coding capacity of 235 codons. The proteins reacting with the anti-U(L)3 antibody form in denaturing polyacrylamide gel bands with apparent M(r)s of 34,000, 35,000, 38,000, 40,000, 41,000, and 42,000 and designated 1 to 6, respectively. Studies on their origins revealed the following. (i) The U(L)3 proteins forming all six bands were present in lysates...