Background: Male infertility has many causes, including genetic infertility. The NOP2/Sun domain family, member7 (Nsun7) gene, which encodes putative methyltransferase Nsun7, has a role in sperm motility. The aim of the present study was to investigate the effect of the T26248G polymorphism on Nsun7 protein function and its role in male infertility. Materials and Methods: Semen samples were col...

Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...

conclusions accordingly, it can be concluded that locally low temperatures and acidic conditions are the main suspected reasons for protein misfolding. results based on the study findings the low temperature, 0°c, and acidic condition change the structure more strongly than other mentioned conditions. the low temperature and acidic conditions seem to have the most misfolding effect on insulin s...

background permanent antigenic variation of influenza viruses causes a major concern to develop an effective human influenza vaccine. conserved antigens are new vaccine candidates because it is not necessary to match the prepared vaccine with circulating strains. ion channel m2 protein is conserved among all influenza a viruses, allowing the virus to enter host cells. objectives to prepare an e...

The study was conducted to determine the effect of varying protein levels and preservation method on external egg qualities of Japanese quails. A total of 180 birds were randomly allotted into three dietary treatments in a completely randomized design (CRD) replicated six times. A factorial arrangement of 3 × 4 × 4 which involves 3 protein levels (15, 20 and 25% crude protein), 4 storage method...

the rotavirus nonstructural protein 4 (nsp4), is a multi functional protein that play key role in both viral morphogenesis and cytopathic effect associated with cell death. however, the complete biological effect of nsp4 remains to be clarified. since to obtain further knowledge about this protein there is a need for recognizing antibody and there is no commercial antibody against this protein...

In this paper, homogeneous, wavelength shift biosensor is designed for sensing the protein concentration using two dimensional Photonic Crystal Ring Resonator (PCRR). The sensor is designed to monitor the protein concentration from 0% to 100%. The proposed sensor is composed of periodic Si rods embedded in an air host with a circular PCRR that is placed between the inline quasi waveguides. It i...

prostate-specific antigen (psa) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a cm-sephadex c-50 and by gel filtration on a sephacryl s-200 column. a single 33-kda protein band appeared in sds-page. high pressure liquid chromatography (hplc) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five diff...

background: recently, botulinum neurotoxin (bont)-derived recombinant proteins have been suggested as potential botulism vaccines. here, with concentrating on bont type e (bont/e), we studied two of these binding domain-based recombinant proteins: a multivalent chimer protein, which is composed of bont serotypes a, b and e binding subdomains, and a monovalent recombinant protein, which contains...