Degradation of insulin was studied in this work. Casein and protamine could obviously suppress degradation of insulin by intestinal enzymes, and could protect insulin from degradation by the mechanism of competition and combination with proteolysis enzyme. What is more, co-incubated with HP-beta-CD-casein or HP-beta-CD-protamine, most insulin was protected from degradation by intestinal enzymes...

Fig. 1 . Effect of increasing amounts of protamine sulfate on the ace measurement of hepann in whole blood (1 USP unlt/mL) The protam,ne/hepann ratio is based on the assumption that 0.01 mg of protamine neutralizes 1 unIt of hepaiin. The measured hepaiin concentration is shown as a percent of anti-factor Xa activity relative to baseline (no added protamine) determined in each experiment. The re...

Surface modifications of poly(lactide-co-glycolide) microparticles with different polycationic electrolytes have mainly been studied for conjugation to antigens and/or adjuvants. However, the in vivo immunological effects of using surface charged particles have not been address yet. In this study, microparticles were coated or not with protamine, a cationic and arginine-rich electrolyte that co...

The aim of this report has been evaluating protamine content and DNA integrity of two patients with globozoospermia undergoing Intra Cytoplasmic Sperm Injection (ICSI). Semen analysis was carried out according to WHO criteria. Protamine deficiency and DNA fragmentation was assessed using Chromomycin A3 and sperm chromatin dispersion assay respectively. ICSI and chemical activation were carried ...

Conclusive evidence has been obtained for the presence of both hepatic and extrahepatic triglyceride lipase activities (TGLA) in rat postheparin plasma, and an assay has been devised for their selective measurement. Heparin-released TGLA in plasma from the intact rat, like TGLA in postheparin hepatic perfusate, was relatively resistant to inactivation by salt and protamine. Postheparin TGLA obt...

The relative importance of heparin-like compounds in mediating vascular repair is unclear. We investigated how protamine, a chelator of heparin, affected endothelial cell inhibition of vascular smooth muscle cell growth and intimal hyperplasia. The 52% ( P < 0.001) reduction in smooth muscle cell proliferation produced by postconfluent endothelial cell-conditioned medium was entirely reversed b...

Injections of heparin had no effect on the dextran anaphylactoid reaction in rats but neutralised the inhibitory action both of protamine and of borate. Heparin, protamine, and borate did not modify the histamine or 5-hydroxytryptamine responses in rat paws and so their effects on dextran may be due to modification of the release mechanisms of these two amines from tissue mast cells. Correspond...