In this study, the complexity of chromatin integrity was investigated in frozen-thawed semen samples from 37 sires with contrasting fertility, expressed as 56-day non-return rates (NR56). Protamine deficiency, thiols, and disulfide bonds were assessed compared previously published data for DNA fragmentation index (DFI) high stainability (HDS). addition, vitro embryo development sperm methylatio...

Several gene expressions are related to the success of spermatogenesis. Protamine plays an important role in sperm DNA packaging, spermatogenesis, and quality. This study aimed isolate RNA from spermatozoa determine expression profiles protamine 1 (PRM1) 2 (PRM2). Six Pasundan bulls aged 5-8 years with a body weight 380 kg-430 kg were used for this study. The classified into group A (70%-79%) B...

introduction: pronuclear size, morphology and orientation are known to affect embryo quality and implantation. the present study was aimed at evaluation of the possible relationship between protamine deficiency with pronuclear morphology and also cleavage and embryo quality. material and methods: semen sample of 160 candidate icsi and ivf couples were analyzed for sperm concentration, and motil...

Double stranded cDNA molecules complementary to purified Rainbow trout protamine mRNA have been cloned in the bacterial plasmid pBR322. In order to circumvent the problems associated with a heterogeneous cDNA probe when identifying recombinants, a comparative hybridisation technique was used which can resolve between closely related cloned sequences. Using this technique, selected recombinants ...

We report the synthesis of a simple pyrene derivative and its application in protamine detection and monitoring of trypsin activity. This assay can be conducted in aqueous solution and features rapid response, visual detection, high sensitivity and selectivity. The limit of detection of protamine was 0.5 μg mL(-1). The IC50 value of a soybean trypsin inhibitor was estimated to be 0.51 U mL(-1).