Lagerstroemia indica is an important woody ornamental plant worldwide. However, the application of many technologies, such as transgenic breeding and genome editing, has been severely hampered due to lack efficient calli induction regeneration technology. Here, we discussed a reliable protocol using whole-leaf explants. This protocol’s effectiveness for systems in crape myrtle were up 70.33% 44...

During somatic embryogenesis of Pinus koraiensis, the ability embryogenic callus to produce embryos gradually decreases with long-term proliferative culture, which seriously affects large-scale application this technology. In study, calli Korean pine 1–100 cell line were used as materials. It was found that in 7-day subculture cycle potential remained for longer time. comparison a 14 days, indo...

The present study was performed to evaluate the effects of different concentrations of ethyl methane sulfonate (0, 0.1, 0.2 and 0.5%) on some physiological characteristics of regenerated plants from calli of stevia at 30, 60 and 120 min under various levels of salinity stress (0, 50 and 100 mM of NaCl). This experiment was carried out based on completely randomized two-factorial designs with th...

A 52 year old woman had autoimmune hepatitis and an increased concentration of serum carbohydrate antigen 19-9 (CA19-9). The origin of the raised CA19-9 was studied using immunohistochemistry. Liver biopsy section showed chronic active hepatitis with large numbers of proliferated bile ductules. Immunohistochemical analysis revealed that the proliferated bile ductule cells were positive for prol...

The aim of this study was to evaluate the membrane integrity and some physiological responses rootstock citrus calli under exposure different concentrations NaCl. Selected salt-tolerant cell lines were compared with salt-sensitive Troyer’s citrange (Citrus sinensis [L.] x Citrus trifoliata Raf.) (TC) respect growth, water content, Na+, K+ Cl? ion content as well stability NaCl concentrations. r...

The popular ornamental plant Petunia is also a valuable model in tissue culture. Cellular conversions during differentiation and regeneration have been investigated using various combinations of phytohormones; however, studies on genes for reprogramming toward desired identities limited. In this study, we isolated protoplasts cultured them the callus, rooting, or shooting stages, which were use...

Cold stress has always been a major abiotic factor affecting the yield and quality of temperate fruit crops. Ethylene plays critical regulatory role in cold response, but underlying molecular mechanisms remain elusive. Here, we revealed that ethylene positively modulates apple responses to stress. Treatment with 1-aminocyclopropane-1-carboxylate (an precursor) aminoethoxyvinylglycine biosynthes...

The effect of abscisic acid (ABA) and excess benzyladenine (BA) on the formation of shoot from tea (Camellia sinensis L. assamica × sinensis) leaf was investigated in this research. Callus was formed and grew well when explants were cultured on LS basal medium supplemented with (in mg/L) thiamine-HCl, 1.25; pyridoxine-HCl, 0.625; nicotinic acid, 0.625; indole-3-acetic acid (IAA), 30; naphthalen...