objective: the aim of the present study was to investigate the effects of four equilibration times (2, 4, 8 and 16 hours) and two extenders (tris or bioxcell®) on cryopreservation of buffalo semen. materials and methods: in this experimental study, split pooled ejaculates (n=4), possessing more than 70% visual sperm motility were divided in two aliquots and diluted in bioxcell ® and tris-citric...

The time required to train rams to an artificial vagina (AV) makes collecting semen from large numbers of rams difficult. To manage this problem, we developed a glass, round-bottomed, 1.9-cm i.d. x 9.8-cm long vaginal collection vial (VCV). Three experiments were conducted to determine whether the VCV affected 1) semen volume per collection, 2) percentage of motile spermatozoa, 3) forward progr...

Epidermal growth factor (EGF) is one of the important cytokines that play a role in fertility. It is known that EGF affects both male and female reproduction, but its effect on sperm parameters is not fully understood. Up to the present, the effect of EGF on ram sperm motility parameters has not been published. We analyzed motility parameters of ejaculates after 24, 48, and 72 hours from the EG...

when considering a patient with dysphagia, an attempt should be made to determine whether the patient has difficulty only with solid boluses (suggestive of mechanical dysphagia) or with liquids and solids (suggestive of a motility dysphagia). lesions such as an oesophageal tumor and external pressure effect from a lung tumor or aberrant vessel can lead to mechanical dysphagia. endoscopy and / o...

background: there are many studies focused on long or short storage time of epididymal sperm (eps) of different species. there are limited studies on preservation or cryopreservation of the domestic goat eps. objective: the aim of the present study was to evaluate the effects of ex vivo cold-storage on freezing of eps from goat (capra hircus). materials and methods: in a split-plot design the c...

the main purpose of this study was to evaluate the influence of the photoperiod on the seminal traits of crossbreed wool-producing rams throughout on year period. for the effect of photoperiod two periods were considered: decreasing daylight length (summer and autumn) and increasing daylight length (winter and spring). for the study 5 baluchi × moghani (bl×mg) and 5 arkharmerino × moghani (am×m...

the aim of this study was to evaluate the effect of bovine serum albomin (bsa) in stallion semen extender in cool condition. after collection, semen samples were pooled and divided into four equal parts. each part was diluted with one of the following extenders: 1) extender without bsa (bsa-0), 2) extender containing 5 mg/ml bsa (bsa-5), 3) extender containing 10 mg/ml bsa (bsa-10), and 4) exte...

The motility characteristics and the behaviour of caput spermatozoa were investigated. After dilution in B2 medium the majority of spermatozoa presented head-to-head agglutination and a twisted flagellum. Only a small population of these spermatozoa developed an anarchic motility pattern. The addition of crude bovine epididymal fluid forward motility protein (FMP) to the incubation medium suppr...

This study was carried out to investigate effects of copper sulphate (CuSO4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and DNA damage, after semen dilution and cryopreservation. Semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. A total number of 25 samples were used in each...

The objective of the present study was to examine the effects of chilling period on semen quality of Sannan and Jamunapari. Semen of goat breeds were collected with artificial vagina and chilled at 5-9˚C. Volume, colour and pH were determined on fresh semen. After dilution, microscopic examination was performed for the progressive motility and sperm viability. Diluted semen of both breed was co...