The spermatozoon of the mealybug Pseudococcus obscurus Essig is a filamentous cell (0.25 micro by 300 micro) which exhibits three-dimensional flagellations throughout most of its length. It has microtubules (200 A diameter) and a threadlike nuclear core (0.07-0.09 micro diameter) which extend almost its entire length, but apparently it has no mitochondria, centrioles, typical flagellum, or acro...

This study compared a number of semen parameters of two separate groups of the common marmoset monkey (Callithrix jacchus) in order to determine the effect of a continuous potentially stressful situation on these parameters, and thus on the monkey's reproductive ability. The semen from 16 adult male marmoset monkeys was collected and analysed to compare semen parameters between a 'normal' (cont...

Kozdrowski R., A. Dubiel, W. Bielas, M. Dzięcioł: Two Protocols of Cryopreservation of Goat Semen with the Use of Computer-Assisted Semen Analysis System.Acta Vet. Brno 2007, 76: 601-604. The objective of the study was a comparison of two protocols of goat semen cryopreservation with the use of computer-assisted semen analysis system. Twenty ejaculates obtained with electroejaculation method we...

BACKGROUND Although the motile sperm organelle morphology examination (MSOME) was developed only as a selection criterion, its application as a method for classifying sperm morphology may represent an improvement in evaluation of semen quality, with potential clinical repercussions. The present study aimed to evaluate individual variations in the motile sperm organelle morphology examination (M...

Capacitation of spermatozoa, a complex sequence of events that render them able to fertilize the egg, is generally associated with a switch from lineal, progressive movement to a vigorous, non-progressive pattern characterized by starlike tracks, a process known as hyperactivation. Development of a method for the analysis of progressive and hyperactive tracks is thus important for the assessmen...

One ejaculate from each Of 13 bulls was used to determine the influence of glycerol level on post-thaw susceptibili ty of spermatozoa in French straws to cold shock. Treatments were arranged factorially and included yolk-Tris and yolk-citrate diluents; 7, 9 and 11% glycerol ;and exposure to 1, 20 or 37 C water baths for 3 rain immediately post-thaw. Percentage of motile spermatozoa and percenta...

BACKGROUND Reactive oxygen species (ROS)-mediated damage to human spermatozoa has been implicated in the pathogenesis of male infertility. Although ROS production by human spermatozoa has been extensively studied, the cell-to-cell variation in ROS production by spermatozoa at different stages of maturation has never been investigated. METHODS In this study, we determined ROS production by sub...

OBJECTIVE The object of present study was to investigate the effects of direct addition of Tribulus terrestris extract on human sperm parameters. DESIGN Semen specimens from 40 healthy men volunteers were divided into 4 groups: one group received no treatment (control group) while the others were incubated with 20, 40, and 50 µg/mL of T terrestris extract (experimental groups). Motility, viab...

Background: Sperm preparation is a fundamental step in ART and attempt for developing new methods to be continued. Objective: To evaluate the efficacy of upstream method for separation of human spermatozoa and its effects on sperm parameters including: motility, morphology, and concentration. Materials and Methods: Semen samples from 17 men who referred to infertility clinic for evaluating thei...