The major nuclear pore protein p62 is modified by O-linked N-acetylglucosamine and functions in nuclear transport. We have cloned, sequenced, and expressed the full-length rat p62 cDNA. The rat p62 mRNA is 2,941 nucleotides long and encodes a protein of 525 amino acids containing 30% serine and threonine residues. The amino acid sequence near the amino-terminus contains unique tetrapeptide repe...

We have determined the complete sequence of chick vinculin from two overlapping cDNA clones. The vinculin mRNA consists of 262 bp of 5' untranslated sequence, an open reading frame of 3195 bp (excluding the initiation codon) and a long 3' untranslated sequence (greater than 2 kb). Chick vinculin contains 1066 amino acid residues, and has a deduced molecular mass of 116,933 Da. Analysis of the d...

The Triple β-Spiral is a novel protein structure that plays a role in viral attachment and pathogenesis. At present, there are two Triple β-Spiral structures with solved crystallographic coordinates – one from Adenovirus and the other from Reovirus. There is evidence that the fold also occurs in Bacteriophage SF6. In this thesis, we present a computational analysis of the Triple β-Spiral fold. ...

Motivation: Development of tools for identification of new thioredoxin-fold proteins as well as other proteins belonging to superfamilies with low primary sequence conservation. Results: We present several algorithms for identifying thioredoxin (Trx)-fold proteins containing a conserved CxxC motif (two cysteines separated by two residues). The low conservation of primary sequence in this protei...

Management of adults with primary immune thrombocytopenia (ITP) has changed dramatically in the past 10 years. New regimens of corticosteroids for first-line treatment have been introduced and are currently being evaluated in a randomized clinical trial. Many patients may not have durable remissions with initial corticosteroid regimens and may require additional, second-line, treatment. For the...

The sequence of the 356 nucleotide residues of chrysanthemum stunt viroid (CSV) has been determined. Overlapping linear viroid fragments were obtained by partial ribonuclease digestion, radiolabelled in vitro at their 5'-ends, and sequenced using partial enzymic cleavage methods. Of the CSV sequence, 69% is contained in the published sequence of potato spindle tuber viroid (PSTV). Differences i...

Two peptides containing the desmosine cross-link were isolated and purified from a subtilisin digest of oxalic acidsolubilized elastin. Molecular weights averaged approximately 6000. Automated sequence data suggest that the desmosines cross-link two polypeptide chains and that both desmosine and isodesmosine are present in the same primary sequence approximately equally substituted. The primary...

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very little is known about lhr and fshr genes of domestic dromedary camels. the main objective of this study was to determine and analyze partial genomic regions of fshr and lhr genes in dromedary camels for the first time. to this end, a total of 50 dna samples belonging to dromedary camels raised in iran were sent for sequencing (25 samples of each gene). we compared the nucleotide sequences ...