Testosterone estradiol-binding globulin (rbTeBG) of rabbit plasma was purified to homogeneity by the sequential use of ammonium sulfate precipitation, affinity chromatography on 5 alpha-dihydrotestosterone-Sepharose, reactive blue-Sepharose, and finally preparative polyacrylamide gel electrophoresis. An overall purification of greater than 3000-fold was achieved with a recovery of 32%. This pur...

Testosterone estradiol-binding globulin (rbTeBG) of rabbit plasma was purified to homogeneity by the sequential use of ammonium sulfate precipitation, affinity chromatography on 5a-dihydrotestosterone-Sepharose, reactive blue-Sepharose, and finally preparative polyacrylamide gel electrophoresis. An overall purification of greater than 3000-fold was achieved with a recovery of 32%. This purified...

Endosomes are prelysosomal organelles that serve as an intracellular site for the sorting, distribution, and processing of receptors, ligands, fluid phase components, and membrane proteins internalized by endocytosis. Whereas the overall functions of endosomes are increasingly understood, little is known about endosome structure, composition, or biogenesis. In this paper, we describe a rapid pr...

Normal human serum has been shown' to exhibit cholesterol-binding properties upon immunoelectrophoresis on agar gel. The evidence clearly supports the concept of a specific type of binding of cholesterol by alpha-beta globulin. Accordingly, the name "transcholesterin" was proposed for the reactive component. In order to evaluate the mechanism of in vitro interaction of cholesterol with transcho...

This paper carried out a comparative analysis of different types of electrophoretic systems which were used for the analysis of casein complex from cow milk (polyacrylamide gel electrophoresis: for the neutral and acidic native conditions, in gradient variant, the presence of sodium dodecyl sulphate or with including urea). Taking into attention the separation efficiency, complexity of electrop...

By making use of preparative field inversion gel electrophoresis, we have constructed a lambda ZAP library that is highly enriched for sequences from the choroideremia locus. In vivo excision of pBluescript SK(-) constructs from lambda ZAP obviates the subcloning of DNA inserts and allows for rapid processing of several hundred recombinants. From a 625 kb Sfil fragment we isolated 7 clones that...

Rat liver clathrin coated vesicles (CVs) were separated into several distinct subpopulations using non-sieving concentrations of agarose, which allowed the separation of species differing primarily in surface charge. Using preparative agarose electrophoresis (Kedersha, N. L., and L. H. Rome, 1986, Anal. Biochem., in press), the CVs were recovered and analyzed for differences in morphology, coat...