An oil painting by Claude Monet, Port-Goulphar, Belle-Ile 1887 (collection of the Art Gallery of New South Wales), was examined to determine both the identity of the pigments used by the artist in this painting and his technique of mixing colors and laying paint on the canvas. The extremely complex construction of the painting was revealed by optical microscopy, scanning electron microscopy (SE...

Among the advanced karyotype analysis approaches embraced by animal cytogenetics during the past decade, chromosome painting has had the greatest impact. Generation of chromosome specific paints is considered pivotal to his development. Additionally, ability to use these paints across species (referred to as Zoo-FISH or comparative painting) is undisputedly the most important breakthrough that ...

For chromosome painting, in situ suppression of repetitive DNA sequences has been well established. Such standard protocols usually require large amounts of Cot-I DNA. Recently, it has become possible to deplete repetitive DNA sequences from library probes by magnetic purification and PCR-assisted affinity chromatography. These "repeat-depleted library probes" appear to be extremely useful for ...

Here we present a simple method for cleaning polyamine human mitotic chromosomes in solution. This was achieved by filtering intact (unburst) nuclei along with both large and small cytoplasmic debris through a series of different pore sized filters. Pure human chromosomes were recovered using a simple reverse filtration step. Fluorescence microscopy was used to validate the chromosome suspensio...

People’s values provide a decision-making framework that helps guide their everyday actions. Most popular methods of assessing values show tenuous relationships with everyday behaviors. Using a new Amazon Mechanical Turk dataset (N = 767) consisting of people’s language, values, and behaviors, we explore the degree to which attaining “ground truth” is possible with regards to such complicated m...

Introduction This sketch presents a method for real-time 3D Sumi-e rendering. Sumi-e is the one of representative art forms of the Black ink painting in Korea, Japan and China. Most of researches about Sumi-e paintings in NPR (Non-Photorealistic Rendering) have focused on 2D or 2.5D Sumi-e brushwork simulation. We suggest a new automated rendering system for 3D image of Sumi-e painting. The fol...

We present algorithms that produce a natural watercolor appearance of 3D-scenes and render them in realtime. Our approach imitates the most important natural effects of watercolor and incorporates two essential painting techniques: the wet-in-wet and the wet-on-dry painting. We apply our technique to different scenes, and show how to segment and abstract the content.

Ours is, at its heart, a profession of service predicated on mutual trust. All too often policies aimed at resolving a particular problem in health care are inadequate not because they fail at what they were intended to do, but rather because of the unintended consequences, both direct and indirect, that are inevitable in complex systems where competing interests preclude unity of purpose. Ulti...