Anti-Müllerian hormone (AMH) is a glycoprotein produced by the granulosa cells of preantral and small antral follicles. AMH concentrations reflect ovarian physiology with high precision, thus serving as a more sensitive marker of the ovarian re-serve than chronological age. This hormone plays a role in the pathogenesis of menstrual disorders and fertility in both obesity and polycystic ovary sy...

This manuscript reviews the present knowledge related to folliculogenesis in sheep.Folliculogenesis starts with formation of primordial follicles before birth, present as a pool containing a certain number of follicles. By the attainment of puberty, a group of follicles from that pool starts to grow, a process known as primordial follicle activation or recruitment. The number of growing primord...

Background: Cryopreservation of oocytes is an effective technology in assisted reproductive technology. Although successful vitrification of gametes has been reported in several mammalian species, the survival is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) on preantral follicle development after vitrifi...

Ovarian follicular development involves continual remodelling of the extracellular matrix (ECM) forming the basement membrane and intercellular framework that support granulosa cell (GC) growth and differentiation. Insight into the molecular regulation of ovarian ECM remodelling is potentially translatable to tissue remodelling elsewhere in the body. We therefore studied the link between a gene...

The aim of the present study was to investigate the effect of follicle stimulating hormone (FSH) on survival, activation and growth of ovine primordial follicles using histological studies. Pieces of ovine ovarian cortex were obtained and cultured for 6 days in Minimum Essential Medium supplemented with or without FSH (50 ng/mL). These fragments were then process to be used for histology compar...

BACKGROUND There is substantial evidence both in humans and in animals that a prolonged reduction in plasma thyroid hormone concentration leads to reproductive problems, including disturbed folliculogenesis, impaired ovulation and fertilization rates, miscarriage and pregnancy complications. The objective of the present study is to examine the consequences of chronic hypothyroidism, induced in ...

Vitrification of preantral follicles is a promising technique to preserve female fertility. The aim the present study was evaluate effect supplementation α-tocopherol in vitrification solution on viability, lipid peroxidation and mRNA expression superoxide dismutases (SOD1 SOD2) vitrified cultured ovine at day-6 day-12. Preantral (200-300 µm) were isolated from ovaries by mechanical method dist...

Background: Polycystic ovary syndrome (PCOS) is a multifactorial, heterogeneous, complex genetic endocrine and metabolic disorder, diagnostically characterized by chronic anovulation, polycystic ovaries and biochemical and clinical manifestations of hyperandrogenism. In patients with PCOS lower apoptotic rates found in granulosa cells compared to women with regular ovulatory cycles and this lea...

To date, methods for quantifying cellular proliferation within the human ovary have relied primarily on static measurements at time of tissue recovery, such as labeling ki67 and phosphohistone-H3. Here, we describe a method based consecutive injection two thymidine analogues, ethynyl deoxyuridine (EdU) 5-chloro-2′-deoxyuridine (CldU), in ovarian xenograft model, allowing resolution division dis...