Summary Understanding lineage specification during human pre-implantation development is a gateway to improving assisted reproductive technologies and stem cell research. Here we employ pseudotime analysis of single-cell RNA sequencing (scRNA-seq) data reconstruct early mouse embryo development. Using time-lapse imaging annotated embryos, provide an integrated, ordered, continuous tran...

Abstract Understanding human peri-implantation development is necessary to improve in vitro fertilisation. Technical progress, such as scRNAseq and integrated stem cell models have dramatically improved our ability understand development. However, in-depth analysis systematic validation embryos remains build a reliable reference. Here, we started by integrating pre- post-implantation embryo dat...

BACKGROUND Embryo implantation is crucial for animal reproduction. Unsuccessful embryo implantation leads to pregnancy failure, especially in human-assisted conception. Environmental factors have a profound impact on embryo implantation. Because people are being exposed to more light at night, the influence of long-term light exposure on embryo implantation should be explored. METHODS The eff...

Failure of the human embryo to implant into the uterine wall during the early stages of pregnancy is a major cause of infertility. Implantation involves embryo apposition and adhesion to the endometrial epithelium followed by penetration through the epithelium and invasion of the embryonic trophoblast through the endometrial stroma. Although gene-knockdown studies have highlighted several molec...

OBJECTIVE To evaluate the effects of a gonadotropin releasing hormone agonist (GnRHa) injection prior to embryo transfer on implantation and pregnancy rate. MATERIALS AND METHODS We performed a retrospective analysis of patients undergoing in vitro fertilization (IVF) therapy with and without GnRHa preinstallation into the uterine cavity just before embryo transfer between January 2012 and Ma...

In polytocous animals, blastocysts are evenly distributed along each uterine horn and implant. The molecular mechanisms underlying these precise events remain elusive. We recently showed that lysophosphatidic acid (LPA) has critical roles in the establishment of early pregnancy by affecting embryo spacing and subsequent implantation through its receptor, LPA3. Targeted deletion of Lpa3 in mice ...

background: recent reports have suggested that ultrasound-guided embryo transfer (ug-et) might improve pregnancy rates. objective: to determine whether transabdominal ug-et is a useful tool for increasing pregnancy and implantation rates in patients undergoing ivf or icsi. materials and methods: a prospective randomized clinical trial was conducted in 180 patients in order to compare embryo tra...

background: implantation is one of the essential steps for the success of assisted reproductive techniques (art). their success depends on three main factors: embryo quality, endometrial receptivity (er), and synchrony between embryo and endometrium. there are various factors that regulate the complex process of implantation. in this regard, one may refer to human chorionic gonadotropin (hcg) a...

OBJECTIVE To describe a patient selection method for elective single embryo transfer (eSET), emphasizing inclusion criteria and results. METHODS This retrospective study included all cases seen in a private clinic between June 2011 and December 2016, in La Paz, Bolivia (3600 meters above sea level). Elective single embryo transfer was the method of choice in 34 IVF/ICSI cycles, all in the bla...