Neutrophil transmigration requires the localization of neutrophils to endothelial cell junctions, in which receptor-ligand interactions and the action of serine proteases promote leukocyte diapedesis. NB1 (CD177) is a neutrophil-expressed surface molecule that has been reported to bind proteinase 3 (PR3), a serine protease released from activated neutrophils. PR3 has demonstrated proteolytic ac...

Luminescent characteristics of K3Lu(PO4)2:Pr3+ (1 and 5 mol.%) microcrystalline powders, a promising optical material for scintillation applications, were investigated using various experimental techniques. The shows emission features connected with both high intensity interconfigurational 4f15d?4f2 transitions (broad UV bands) intraconfigurational 4f2?4f2 (weak lines in the visible range). out...

Caspase-3-mediated spontaneous death in neutrophils is a prototype of programmed cell death and is critical for modulating physiopathological inflammatory responses; however, the underlying regulatory pathways remain ill defined. Here we determined that in aging neutrophils, the cleavage and activation of caspase-3 is independent of the canonical caspase-8- or caspase-9-mediated pathway. Instea...

INTRODUCTION Proteinase 3 (PR3) is released from neutrophil azurophilic granules and exerts complex effects on the inflammatory process. PR3 catalyzes the degradation of a number of macromolecules, but the consequences on blood cells are less well defined. In the present study, the effect of PR3 on human platelets was thoroughly investigated. METHODS The experiments were performed on washed p...

Myeloid leukemia cells, the human promyelocytic cell line HL-60, and a subpopulation of normal marrow cells produce a leukemia-associated inhibitor (LAI) that reversibly downmodulates DNA synthesis of normal granulopoietic progenitor cells colony-forming unit granulocyte-macrophage (CFU-GM). We isolated an active 125-kD component of LAI from HL-60 conditioned medium (CM), subjected it to cyanog...

Neutrophils are the first responders of the inflammatory response. They are characterized by their potent cytotoxic content but also by their limited lifetime. This short half-life is thought to be a self-protecting mechanism for the host, as highlighted by the numerous pathologies associated with imbalanced neutrophil survival. Neutrophil spontaneous death is the prototype of programmed cell d...

Three different compounds, cubic Gd2O3, orthorhombic GdGaO3, and Gd3Ga5O12 doped with Pr3+ ions were fabricated as waveguiding films by pulsed laser deposition from the same target material, (Pr:GGG) single crystal. All of them deposited at substrate temperature 800 °C. The crystalline phases obtained depend only on ambient oxygen pressure type (YAG or YAP crystals). structural texture properti...

Magnetic nanoparticles offer some attractive possibilities in biomedicine because it has the special physical properties. Magnetic hyperthermia using magnetic nanoparticles requires that the magnetic particles have not only high heating ability in low magnetic field but also to be non-toxic for biomedical use. Although the reports of bulk magnetic materials or Fe3O4 nanoparticles have known for...