Membrane vesicles isolated from Azotobacter vinelandii strain O by lysis of spheroplasts in potassium of sodium phosphate buffer develop a transmembrane electric potential during respiration. The magnitude of this potential was determined by three independent methods: (i) fluorescence of 3,3'-dipropylthiodicarbocyanine and 3,3'-dihexyloxacarbocyanine; (ii) uptake of 86Rb+ in the presence of val...

We assessed the changes in arterial potassium concentration during exercise and recovery in relation to exercise tolerance in patients with impaired exercise tolerance. Sixteen patients with cardiac disease were subjected to a cardiopulmonary exercise test on a cycle ergometer. Arterial potassium and lactate concentrations were measured every minute during and after exercise, and ventilatory th...

in order to study the effects of simultaneous application of znso4 and biological fertilizer, azotobacter chroococcum and azospirillum brasilense, on grain yield and nitrogen uptake efficiency in rapeseed (brassica napus l.), cv. hyola308, a field experiment was conducted as split plot factorial based on randomized complete block design at research field of faculty of agricultural sciences, uni...

Oxygen uptake is essential for active ion transport across the isolated silkworm midgut as well as across other isolated epithelial membranes such as the frog skin and toad bladder. The study of the chemical relationship between these two processes in the skin and bladder has been rewarding. The short-circuit current across the isolated frog skin and toad bladder is accounted for entirely by th...

PURPOSE Experiments were conducted to test whether protein kinase C activation causes changes in active sodium-potassium transport in an established SV-40 transformed line (ODM2) of cultured human nonpigmented ciliary epithelial cells. METHODS Rubidium-86 (86Rb) uptake was measured and the data used to determine the rate of potassium entry into the cells. RESULTS Protein kinase C activator,...

To evaluate the possibility that an apical membrane conductive pathway for oxalate is present in the rabbit distal ileum, we studied oxalate ([14C]oxalate) and chloride (36Cl) uptake into brush-border membrane vesicles enriched 15- to 18-fold in sucrase activity. Voltage-sensitive pathways for oxalate and chloride were identified by the stimulation of uptake provided by an inwardly directed pot...