OUR knowledge of the role of glucose in the metabolism of the heart in the whole animal appears to be restricted to its extraction from circulating blood and its effect on glycogen elevation, despite extensive studies in isolated tissue and organ preparations. In this study the myocardial ''.oritent of high energy phosphate compounds, estimated as acid-labile phosphate, was examined following i...

ATP induced swelling of isolated yeast mitochondria suspended in an isoosmotic solution of potassium gluconate. Valinomycin stimulated the swelling rate, indicating that K+ influx in the presence of ATP is rate-controlling. This swelling was inhibited by ADP, phosphate (probably acting on the external face of the inner membrane), and Mg2+, which forms a complex with ATP. ATP-induced swelling di...

Eight normotensive subjects were studied in a randomized crossover trial of a high calcium diet (1800 mg of calcium/day) for a week against a low calcium diet (200 mg of calcium/day) for a further week. The subjects were placed on a diet containing 200 mg of calcium/day throughout the study and the high calcium diet was achieved by supplementing the low calcium diet with calcium glubionate and ...

Staphylococcus aureus organisms, grown in basal medium of water activity (a,) 0.993 and in basal medium adjusted to several different a, values by addition of NaCl, were analysed for the following components: sodium, potassium, calcium, magnesium, total amino acids, inorganic phosphate, chloride, water, DNA, RNA, protein. Cell water decreased from 1-66 to 0.83 g./g. dry weight when a, was decre...

Nitrite and nitrate are main stable products of nitric oxide, a pivotal cellular signaling molecule, in biological fluids. Therefore, accurate measurement of the two ions is profoundly important. Nitrite is difficult to be determined for a larger number of interferences and unstable in the presence of oxygen. In this paper, a simple, cost-effective and accurate HPLC method for the determination...

Phosphoenolpyruvate (PEP) carboxylase was purified over 400-fold from Plasmodium berghei. The purified enzyme was stable in 0.4 m potassium phosphate buffer (pH 7.4) containing 0.5 m glucose, 1 mm ethylenediaminetetraacetic acid (EDTA), and 1 mm MgCl(2). It had a molecular weight of 280,000 determined by sucrose density gradient centrifugation in this buffer, but it aggregated and was unstable ...

There is little information regarding kidney function in patients with beta-thalassemia minor. In this study we investigated kidney function tests in 50 children with beta-thalassemia minor (22 boys and 28 girls). Twenty-four-hour urine samples were collected and analyzed for sodium, potassium, calcium, magnesium, creatinine, phosphate, uric acid, protein, and beta2-microglobulin. Blood samples...

losartan potassium, valsartan , telmisartan and irbesartan are angiotensin-ii-receptor antagonists (ara ii) group which used in treatment of hypertension alone or in combination with other drugs mainly hydrochlorothiazide. rp- hplc method was developed for the assay of three angiotensin-ii-receptor antagonists (ara-iis) in presence of hydrochlorothiazide. the method was performed by reversed ph...