The circadian rhythm of the urinary saturation of uric acid, sodium acid urate, and ammonium acid urate was studied in 5 normal healthy male volunteers before and during 5 days of treatment with sodium-potassium citrate (1 g t.i.d., 1 g q.i.d., or 3 g t.i.d.). Urinary saturation was estimated on the relative supersaturation scale of Marshall and Robertson. Uric acid relative supersaturation var...

Blood pressure (BP) shows a continuous relationship with the risk of CVD. There is substantial evidence that dietary potassium exerts an anti-pressor effect. Most clinical trials have used KCl. However, the chloride ion may have a pressor effect and in foods potassium is associated with organic anions. In a double-blind randomized placebo-controlled trial we explored the effect on BP of two sal...

Phosphoenolpyruvate (PEP) carboxylase was purified over 400-fold from Plasmodium berghei. The purified enzyme was stable in 0.4 m potassium phosphate buffer (pH 7.4) containing 0.5 m glucose, 1 mm ethylenediaminetetraacetic acid (EDTA), and 1 mm MgCl(2). It had a molecular weight of 280,000 determined by sucrose density gradient centrifugation in this buffer, but it aggregated and was unstable ...

The B-IV and B-IX zonal ultracentrifuge rotors were applied to the concentration and purification of the Moloney and Rauscher murine leukemia viruses from large volumes of infected tissue culture fluids and animal materials. Potassium tartrate, potassium citrate and sucrose gradients were used to obtain viral concentrates from the density 1.16 to 1.18 zone. Proteolytic enzyme digestion of tissu...

Objectives: The objectives of this study were to the various biochemical changes occurring in a stored whole blood unit at day 0, 17th, and 35th full bag containing citrate phosphate dextrose adenine-1 anticoagulant. Methods: A cross-sectional descriptive had been carried out among 110 healthy volunteer donors Department Transfusion Medicine, RNT Medical College, Udaipur, between August 2019 Ju...

The use of bovine LF as ingredient in infant formulas to mimic human milk composition is great interest for the food industry; however, thermal treatments may induce denaturation LF, reducing its nutritional benefits. In this study, effect four salts, authorized additives (potassium phosphate (PP), sodium (SP), potassium citrate (PC) and (SC)), stability was studied. concentration soluble after...