نتایج جستجو برای: pore system

تعداد نتایج: 2266274  

Journal: :Cell 2011
Stefan Amlacher Phillip Sarges Dirk Flemming Vera van Noort Ruth Kunze Damien P. Devos Manimozhiyan Arumugam Peer Bork Ed Hurt

Despite decades of research, the structure and assembly of the nuclear pore complex (NPC), which is composed of ∼30 nucleoporins (Nups), remain elusive. Here, we report the genome of the thermophilic fungus Chaetomium thermophilum (ct) and identify the complete repertoire of Nups therein. The thermophilic proteins show improved properties for structural and biochemical studies compared to their...

Journal: :Cell 1996
Symeon Siniossoglou Christian Wimmer Michael Rieger Valerie Doye Hildegard Tekotte Chris Weise Sonja Emig Alexandra Segref Edward C Hurt

In a genetic screen for nucleoporin-interacting components, a novel nuclear pore protein Nup84p, which exhibits homology to mammalian Nup107p, was isolated. Nup84p forms a complex with five proteins, of which Nup120p, Nup85p, Sec13p, and a Sec13p homolog were identified. Upon isolation of Sec13p-ProtA, nucleoporins were still associated, but the major copurifying band was a 150 kDa protein, sho...

Journal: :Current opinion in cell biology 2012
Javier Fernandez-Martinez Michael P Rout

Macromolecular assemblies can be intrinsically refractive to classical structural analysis, due to their size, complexity, plasticity and dynamic nature. One such assembly is the nuclear pore complex (NPC). The NPC is formed from ∼450 copies of 30 different proteins, called nucleoporins, and is the sole mediator of exchange between the nucleus and the cytoplasm in eukaryotic cells. Despite sign...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2011
Kimihisa Yoshida Hyuk-Soo Seo Erik W Debler Günter Blobel André Hoelz

So far, only a few of the interactions between the ≈ 30 nucleoporins comprising the modular structure of the nuclear pore complex have been defined at atomic resolution. Here we report the crystal structure, at 2.6 Å resolution, of a heterotrimeric complex, composed of fragments of three cytoplasmically oriented nucleoporins of yeast: Nup82, Nup116, and Nup159. Our data show that the Nup82 frag...

Journal: :journal of advanced materials and processing 0
maryam shojaie bahaabad department of chemical and materialas engineering, shahrood university of technology, shahrood, iran

in this work, sio2 –zro2 mixed oxides was prepared by the polymeric sol–gel route. the characterization of pore structure, which determines the permeation process of membrane, is of great importance. so far, most investigations have focused on such pore structure as specific surface area and pore size distribution, but the surface fractal, the important parameter reflecting the roughness of por...

2011
N. Takei T. Nakano F. Arai

We have developed a unique multicellular aggregate formation platform that utilizes a maskless gray-scale photolithography. The cellular aggregate formed using this platform has a toroidal-like geometry and includes a micro-pore that facilitates the supply of the oxygen and growth factors and expels waste products. As a result, this platform was capable of rapidly producing hundreds of multicel...

Journal: :journal of water sciences research 2013
j bazargan h.r eskandari

filter is one of the main components of embankment dams. by a simple but effective performance, filter protects the dam against erosion and soil scouring in impervious core caused by leakage (piping) and makes it safe. interaction between filter and erodible base soil is a complex phenomenon which is dependent upon several factors, and has challenged researchers for better understanding the fil...

Journal: :The Journal of Cell Biology 1976
N Dwyer G Blobel

A modified procedure for the isolation of a nuclear pore complex-lamina fraction from rat liver nuclei is described. Evidence is provided that the isolated lamina, a 150-A thick, proteinaceous structure, apposes the inner nuclear envelope membrane, connecting nuclear pore complexes and surrounding the entire nucleus.

Journal: :Current Biology 2012
Maureen A. Powers Douglass J. Forbes

The massive nuclear pore complex mediates nucleocytoplasmic traffic ranging from a single histone to a viral genome. To date, dissecting mechanism has been more an exercise in prediction than biochemical certainty. A recent study combines recombinant proteins with nuclei reconstituted in vitro to test predictions in a startlingly productive manner.

Journal: :Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 2016
Hirokazu Watanabe Ryuji Kawano

This study describes the pore-forming properties of magainin 1 in planar lipid bilayers. These bilayers were prepared by the droplet contact method, which was executed on a microfabricated device for a high-throughput study. We arrayed four droplet chambers parallelly in the single device, and the current measurements were carried out simultaneously. Using this system, we measured the channel c...

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