By using a benzethonium chloride concentration 12-fold that described originally (Clin Chem 1979;25:1317-9), we developed a reliable method suitable for routine measurement of total protein in cerebrospinal fluid. Only 10 microL of sample is required. Reactivity to immunoglobulin G (IgG) and to albumin (Alb) is similar, as is necessary for specimens that can have very varied IgG/Alb ratios. The...

immunodetection is performed. Use of the Criterion Stain Free system eliminates the need for the timeand reagentconsuming traditional methods such as Ponceau S staining used to asses protein transfer prior to detection in western blotting applications. We also demonstrate that, while binding of some monoclonal antibodies may be affected by the Criterion Stain Free method, we have not observed a...

Siastatin B analogues, optically active 2-(trifluoroacetamide)- 3,4,5-trihydroxypiperidines having nitromethyl, aminomethyl and carboxyl branched groups at C-5, and (+)-(2R,3R,4R,5R)-5-(aminomethyl)-3,4,5-trihydroxy-2- (hydroxyacetamido)piperidine have been obtained total synthetically from D-ribono-1,4-lactone. Some analogues have inhibitory activity against some glycosidases, and (+)-(2R,3R,4...

Purpose The purpose of this paper is to examine how public sector organizations become nimbler while retaining their resilience during digital transformation. Design/methodology/approach study adopts a hermeneutic approach in conducting deep expert interviews with 22 senior executives and managers multiple organizations. method blends theory views transformation the context enterprise informati...