conclusions based on our study, plca and hlya played a key role in the virulence determination of l. monocytogenes. data analysis also showed that l. monocytogenes could be a causative agent of abortion in pregnant women. results out of 96 samples, 16 isolates of l. monocytogenes by pcr (plca and hlya) and four isolates by culture were identified. there was a significant difference between pcr ...

results using these two sets, we identified h1069q mutation in four patients, c.2335t > g mutation in three, c.3061-1g > a splice site mutation in five, c.3305t > c mutation in one, and c.3809a > g mutation in two patients. conclusions the multiplex arms assay used in this study can be an efficient, reliable, and cost effective method as a primary screen for patients with wilson disease. patien...

abstract        background and objective: chabahar is in southern iran located near the iran-pakistan border. since leishmaniasis is an emerging disease in this region, this study aimed to diagnose the disease and identify different species of leishmania parasite in the patients referred to the central laboratory.       methods: this descriptive cross-sectional study was conducted in 2011-2012 ...

background and objective: the outcome of hepatitis b virus (hbv) infection may be influenced by host factors like human leukocyte antigen (hla). we have investigated hla-a and drb1 alleles in patients with persistent hepatitis b infection compared to subjects who had spontaneously recovered from hbv infection. to complete the findings of this study we performed another survey in certain hla all...

background: toxoplasmosis is one of the most important food-borne diseases distributed worldwide. in order to determinate the involvement of poultry meat in clinical toxoplasmosis, this study aimed to genetic characterization of toxoplasma gondii in tissue samples of serologically positive chickens of tunisia using polymerase chain reaction (pcr). methods: trail of isolation of local t. gondii ...

background: cytochrome p450 2c19 (cyp2c19) is important in metabolism of wide range of drugs. cyp2c19*17 is a novel variant allele which increases gene transcription and therefore results in ultra-rapid metabolizer phenotype (urm). distribution of this variant allele has not been well studied worldwide. the aim of present study was to investigate allele and genotype frequencies of cyp2c19*17 in...

background and aims: canine distemper (cd) is a deadly infectious disease of canidae family. cd is a multi-systemic viral disease and is specified by wide range of clinical symptoms. the manifestations are not always indicative of cd, therefore a laboratory confirmation is necessary for suspected cases. materials and methods: different clinical specimens of 19 cd suspected unvaccinated dogs wer...

Abstract Background We aimed to investigate the association of initial chest CT scan findings with status and adverse outcomes COVID-19 (including ICU admission, mortality, disease severity). This retrospective cohort study was performed in three hospitals Babol, northern Iran, between February March 2020. Cases were confirmed by real-time polymerase chain reaction (RT-PCR). Clinical paraclinic...

The definitive diagnosis of lyssavirus infection (including rabies) in animals and humans is based on laboratory confirmation. The reference techniques for post-mortem rabies diagnosis are still based on direct immunofluorescence and virus isolation, but molecular techniques, such as polymerase chain reaction (PCR) based methods, are increasingly being used and now constitute the principal tool...