Lactobacillus reuteri, a Gram-positive bacterial species inhabiting the gastrointestinal tract of vertebrates, displays remarkable host adaptation. Previous mutational analyses of rodent strain L. reuteri 100-23C identified a gene encoding a predicted surface-exposed serine-rich repeat protein (SRRP100-23) that was vital for L. reuteri biofilm formation in mice. SRRPs have emerged as an importa...

A quartz crystal microbalance with dissipation monitoring (QCMD) has been used to monitor the adsorption and structure of lysozyme monolayers and multilayers, and poly-L-lysine (PLL)-polygalacturonic acid (PGalA) multilayers at a solid-liquid interface using freshly-cleaved mica as a substrate. QCMD measurements were complemented with atomic force microscopy (AFM). AFM images revealed that lyso...

Pectinophiles are bacteria that utilize pectin and only a few related compounds as substrates. Obligately anaerobic pectinophiles have been isolated from the intestinal tracts and gingivae of humans and from the rumina of cattle. We isolated three strains of pectinophilic bacteria from colonic contents of pigs but were unable to isolate pectinophiles from the rumen contents of four sheep, even ...

A study was carried out on a new strain of Rhizopus stolonifer RCH7 isolated from a spontaneouslyfermenting rice husk sample. Growth characteristics on and utilization profile of various substrates by this isolate were investigated. The isolate was found to display the most extensive mycelia proliferation at a temperature of 37°C and a pH of 4.0. Divalent ionic additions did not cause an increa...

rhamnogalacturonan (RG), which were composed of the diglycosyl repeating "nit, -4)-or-D-GalpA-(1-2)-a-LRhap-(1-. The side chains of B-1,4-galactans, branched with fucose and arabinose residues, were linked to the C-4 side of rhamnose residues in the RG regions. The degree of po]ymerization (dps) of GN, which linked the RG regions together, was estimated to be about 4-10 residues, and some were ...

BREED, R. S., -MURRAY, E. G. D., AND HITCHENS, A. P. 1948 Bergey's miianual of deterininative bacteriology, Ed. 6. The Williams & Wilkins Co., Baltimore, Maryland. ,BU7RRI, R. 1902 Zur Isolierung der Anaeroben. Zentr. Bakteriol. Parasitenk., Abt. II, 8, 534-537. CARRE, M. H. AND HAYNES, D. 1922 The estimation of pectin as calcium pectate and the application of this method to the determination o...

125I-Hyaluronic acid (HA) uniquely modified only at the reducing end (Raja, R.H., LeBoeuf, R. D., Stone, G.W., and Weigel, P.H. (1984) Anal. Biochem. 139, 168-177) binds specifically to rat liver endothelial cells in suspension or in culture. About 67-85% of the HA binding sites in isolated cells in suspension and 50% in cultured cells were intracellular, since they were exposed after permeabil...

Pectate lyase 10A (Pel10A) enzyme from Pseudomonas cellulosa is composed of 649 residues and has a molecular mass of 68.5 kDa. Sequence analysis revealed that Pel10A contained a signal peptide and two serine-rich linker sequences that separate three modules. Sequence similarity was seen between the 9.2 kDa N-terminal module of Pel10A and family 2a carbohydrate-binding modules (CBMs). This N-ter...

Taylor, R. J., and Secor, G. A. 1988. An improved diffusion assay for quantifying the polygalacturonase content of Erwinia culture filtrates. Phytopathology 78:1101-1103. The agar diffusion procedure for quantifying pectolytic enzyme activity punched in the agarose with a # 1 cork borer. After incubation at 37 C for 17 was modified to optimize assay sensitivity and simplify its implementation. ...