A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas alpha- and beta-tubulins; we also domonstrate how the procedure can detect minor antibody species in an antiserum dir...

A protein purification procedure using sonication extraction from polyacrylamide gels (PAGE), which involves identification of a particular protein band and its excision, homogenization, sonication, and subsequent passage through a Sephadex G-25 minicolumn, is reported. Our results show a high degree of recovery regardless of the nature of the protein (soluble or membrane bound) or the characte...

2,2,2-Trichloroethanol (TCE) incorporated into polyacrylamide gels before polymerization provides fluorescent visible detection of proteins in less than 5min of total processing time. The tryptophans in proteins undergo an ultraviolet light-induced reaction with trihalocompounds to produce fluorescence in the visible range so that the protein bands can be visualized on a 300-nm transilluminator...

1Plant Molecular and Cellular Genetics Lab, School of Agriculture and Home Economics, Tuskegee University, Tuskegee, Alabama 36088-1614; 2USDA/ARS, Plant Introduction Station, Griffin, Georgia 30223; 3Department of Plant Pathology, Auburn University, Alabama 36849 Agarose is the preferred matrix for resolving DNA fragments resulting from PCR amplifications using arbitrary primers for the detect...

in the present study, we investigated total cell protein patterns of ten isolates of epidermophyton floccosum var.pigmented by sds-page on 10% polyacrylamide resolving gels. densitometric analysis of the gels allowed to detect more than 31 clearly detectable mycelial protein bands with molecular weights in the range of 12 to 98 kd proteins of 12.5, 13.5, 14.4, 16, 18.4, 19.7, 20.1, 23.5, 26, 27...

A hybridoma cell line was isolated which produced monoclonal antibody to one protein component of a yeast-phase cytoplasmic antigenic complex of Blastomyces dermatitidis. The immunoglobulin M antibody product was characterized by immunodiffusion, autoradiography of polyacrylamide gels, and cellulose acetate electrophoresis. By attaching the antibody to an affinity gel, one major protein band wa...

Excessive water production impacts the economics of petroleum reservoir and often leads to their premature shut-in. Production high saline represents a major corrosion for casing, tubing, flow lines, facilities. Polymer solution has been used control plug in high-temperature reservoirs. Most these polymer solutions consist polyacrylamide-based an organic or inorganic cross linker. Polyethylene m...