نتایج جستجو برای: poly beta hydroxybutyrate

تعداد نتایج: 267464  

Journal: :Applied and environmental microbiology 1999
D Kolibachuk A Miller D Dennis

The polyhydroxyalkanoic acid synthase gene from Chromobacterium violaceum (phaC(Cv)) was cloned and characterized. A 6.3-kb BamHI fragment was found to contain both phaC(Cv) and the polyhydroxyalkanoic acid (PHA)-specific 3-ketothiolase (phaA(Cv)). Escherichia coli strains harboring this fragment produced significant levels of PHA synthase and 3-ketothiolase, as judged by their activities. Whil...

2014
H.M. Chang Z.H. Wang H.N. Luo M. Xu X.Y. Ren G.X. Zheng B.J. Wu X.H. Zhang X.Y. Lu F. Chen X.H. Jing L. Wang

Development and selection of an ideal scaffold is of importance for tissue engineering. Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) is a biocompatible bioresorbable copolymer that belongs to the polyhydroxyalkanoate family. Because of its good biocompatibility, PHBHHx has been widely used as a cell scaffold for tissue engineering. This review focuses on the utilization of PHBHHx-base...

Journal: :Journal of bioscience and bioengineering 2006
Suchada Chanprateep Songsri Kulpreecha

The production of the terpolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate), P(3HB-co-3HV-co-4HB), by Alcaligenes sp. A-04 was investigated to determine the superior biodegradable polymer properties over those of poly(3-hydroxybutyrate), P(3HB), and its copolymers. The highest terpolymer content of 68% (w/w) was produced by Alcaligenes sp. A-04 at 60 h by shake-flask cult...

Journal: :FEMS microbiology letters 2015
Margreet J Oosterkamp Sjef Boeren Siavash Atashgahi Caroline M Plugge Peter J Schaap Alfons J M Stams

Alicycliphilus denitrificans strain BC grows anaerobically on acetone with nitrate as electron acceptor. Comparative proteomics of cultures of A. denitrificans strain BC grown on either acetone or acetate with nitrate was performed to study the enzymes involved in the acetone degradation pathway. In the proposed acetone degradation pathway, an acetone carboxylase converts acetone to acetoacetat...

Journal: :Stroke 1984
J R Kirsch L G D'Alecy

Glucagon has been shown previously to increase further the enhanced tolerance for hypoxia observed in mice with elevated blood ketones. Glucagon is also known to increase blood glucose and to alter directly the metabolism of some (liver) cells. Both the increase in blood glucose and altered cellular metabolism could contribute to the increase in tolerance for hypoxia observed in mice given gluc...

Journal: :Biotechnology and bioengineering 2000
H E Valentin S Reiser K J Gruys

To provide 4-hydroxybutyryl-CoA for poly(3-hydroxybutyrate-co-4-hydroxybutyrate) formation from glutamate in Escherichia coli, an acetyl-CoA:4-hydroxybutyrate CoA transferase from Clostridium kluyveri, a 4-hydroxybutyrate dehydrogenase from Ralstonia eutropha, a gamma-aminobutyrate:2-ketoglutarate transaminase from Escherichia coli, and glutamate decarboxylases from Arabidopsis thaliana or E. c...

Journal: :The Biochemical journal 1969
H A Krebs P G Wallace R Hems R A Freedland

1. The rates of formation of acetoacetate and beta-hydroxybutyrate by the isolated perfused rat liver were measured under various conditions. 2. The rates found after addition of butyrate, octanoate, oleate and linoleate were about 100mumoles/hr./g. wet wt. in the liver of starved rats. These rates are much higher than those found with rat liver slices. 3. The differences between the rates give...

2010
Abd-Elghany Hefnawy Seham Youssef Saad Shousha

Pregnancy toxemia was induced in nine pregnant goat does with twins by the stress of fasting with access to water in late pregnancy to investigate the effect of pregnancy toxemia on immunoglobulins (IgA, IgM, and IgG), cortisol, insulin, thyroid, and growth hormones and their correlations with the plasma levels of glucose and beta-Hydroxybutyrate. Plasma samples were collected at 0, 12, 24, 36,...

Journal: :American journal of physiology. Endocrinology and metabolism 2002
G Blomqvist M Alvarsson V Grill G Von Heijne M Ingvar J O Thorell S Stone-Elander L Widén K Ekberg

Using R-beta-[1-(11)C]hydroxybutyrate and positron emission tomography, we studied the effect of acute hyperketonemia (range 0.7-1.7 micromol/ml) on cerebral ketone body utilization in six nondiabetic subjects and six insulin-dependent diabetes mellitus (IDDM) patients with average metabolic control (HbA(1c) = 8.1 +/- 1.7%). An infusion of unlabeled R-beta-hydroxybutyrate was started 1 h before...

Journal: :Transactions of the American Clinical and Climatological Association 2003
George F Cahill Richard L Veech

D-beta-hydroxybutyrate, the principal "ketone" body in starving man, displaces glucose as the predominating fuel for brain, decreasing the need for glucose synthesis in liver (and kidney) and accordingly spares its precursor, muscle-derived amino acids. Thus normal 70 kg. man survives 2-3 months of starvation instead of several weeks, and obese man many months to over a year. Without this metab...

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