Glycerol, a byproduct of the biodiesel industry, can be used by bacteria as an inexpensive carbon source for the production of value-added biodegradable polyhydroxyalkanoates (PHAs). Burkholderia cepacia ATCC 17759 synthesized poly-3-hydroxybutyrate (PHB) from glycerol concentrations ranging from 3% to 9% (v/v). Increasing the glycerol concentration results in a gradual reduction of biomass, PH...

The present study encomparies of the isolation and production of PHB (Poly-β-hydroxybutyrate) producing Bacillus sp., from soil samples. The accumulation of PHB as granules in the organism was analyzed by Sudan black staining method. The PHB production was determined from the selected strains (B6, B7, B8, B10 and B11) by crotonic acid extraction method using U-V spectrophotometer at 235nm. The ...

We report herein on the application of Raman spectroscopy to the rapid quantitative analysis of polyhydroxyalkanoates (PHAs), biodegradable polyesters accumulated by various bacteria. This theme was exemplified for quantitative detection of the most common member of PHAs, poly(3-hydroxybutyrate) (PHB) in Cupriavidus necator H16. We have identified the relevant spectral region (800-1800 cm-1) in...

Glycerol (G), polyglycerols (PGs), glycerol triacetate (GTA), and tributyrate (GTB) were studied as additives of poly(3-hydroxybutyrate) (PHB). The effect different concentrations (5–30%) these compounds on microstructure final properties PHB films evaluated. Microscopic studies revealed that samples with GTA GTB presented uniform microstructure, meanwhile those containing G PGs evidenced exuda...

Oxygen supply implies higher production cost and reduction of maximum theoretical yields. Thus, generation fermentation products is more cost-effective. Aiming to find a key piece for the (poly)-3-hydroxybutyrate (PHB) as product, here we characterize an acetoacetyl-CoA reductase, isolated from Candidatus Accumulibacter phosphatis-enriched mixed culture, showing (kcatNADH/KMNADH)/(kcatNADPH/KMN...

Recombinant Escherichia coli XL1-Blue harboring a high-copy-number plasmid containing the Alcaligenes eutrophus polyhydroxyalkanoate synthesis genes could efficiently synthesize poly(3-hydroxybutyrate) (PHB) in a complex medium containing yeast extract and tryptone but not in a defined medium. One of the reasons for the reduced PHB production in a defined medium was thought to be severe filamen...

BACKGROUND AND PURPOSE Polyhydroxyalkanoates (PHAs), biopolyesters of hydroxyl fatty acids, are synthesized and deposited as cytoplasmic inclusions in many bacteria. We isolated a poly-beta-hydroxybutyrate (PHB)-producing bacterium designated Rhodobacter sphaeroides FJ1. To characterize PHB biosynthesis in this organism, we isolated the genes encoding proteins involved in PHB metabolism. METH...