Abbreviations: A/C: Area in Charges; AFP: Acute Flaccid Paralysis; AFP: Acute Flaccid Paralysis; CNIC: Computerized National Identity Card; cVDPV2: Circulating Vaccine Derived Polio Vaccine Virus type 2; DDM: Direct Dis-imbursement Method; DEWS: Disease Early Warning System; DHMT: District Health Management Team; DHMT: District Health Management Team; DHOs: District Health Offices; DPT: Diphthe...

This study demonstrates that elution of enteroviruses from a mixture of primary- and activated-sludge solids with beef extract at pH 9.2 +/- 0.2 may be less efficient than elution with beef extract at pH 7.2 +/- 0.2 and that elution of enteroviruses from extended-aeration-sludge solids with beef extract is at best no more efficient at pH 9.2 +/- 0.2 than at pH 7.2 +/- 0.2. Thus, the common prac...

introduction: immunization is one of the most important health programs in first level prevention and is also one of the most cost-effective prevention programs in the entire world. this study evaluates the situation of immunization in fewer than one year old afghan refugee children in kerman, iran. materials and methods: this was a cross-sectional study. data was extracted from the records of ...

Global importance of the health condition today: today, polio is on the verge of being erased from the globe. as recently as 988, 25 countries were endemic for polio, with an estimated 350,000 cases. through the first half of 2006, just four countries were reported endemic for polio, and fewer than 700 cases were reported worldwide. this dramatic reduction is the result of massive oral polio va...

Although most enterovirus infections are not serious enough to be life threatening, several enteroviruses such as enterovirus 71 are responsible for severe, potentially life-threatening disease. The epidemic patterns of enteroviruses occur regularly during the year, but they may change due to environmental shifts induced by climate change due to global warming. Therefore, enterovirus epidemiolo...

The study represents the genetic overview of non-polio enteroviruses (NPEV) isolated from acute flaccid paralysis (AFP) cases in Yunnan Province from 2006 to 2010. Molecular typing based on VP1 nucleotide sequence was carried out on 98 NPEV isolates, and 33 serotypes were identified. EV-B was detected most frequently with an overall prevalence of 71.4%, followed by EV-A (18.4%) and EV-C (10.2%)...

BACKGROUND Acute flaccid paralysis (AFP) can be caused by a number of conditions. A common preventable cause is poliomyelitis which is still being reported in Pakistan, Guillain Barre Syndrome (GBS), also known as Acute Inflammatory Demyelinating Polyneuropathy, is another common cause of acute flaccid paralysis. It is important to recognize GBS in childhood as parents consider all acute flacci...

Human rhinovirus (HRV) is the major aetiology of respiratory tract infections. HRV viral load assays are available but limitations that affect accurate quantification exist. We developed a one-step Taqman assay using oligonucleotides designed based on a comprehensive list of global HRV sequences. The new oligonucleotides targeting the 5'-UTR region showed high PCR efficiency (E = 99.6%, R2 = 0....

Monoclonal antibodies (MoAbs) were generated following immunization of Balb/C mice with adenovirus type 5 grown in Hep2 cell line. Six clones reactive to hexon antigen of the virus were stabilized, of which 4 had mu-heavy chain specificity and 2 were of gamma-heavy chain type. Three of the clones (ADV-1, ADV-3 and ADV-5) had a high ELISA reactivity to the hexon antigen but exhibited differentia...