Short oligonucleotides binding complementary mRNA essential for bacterial growth could be used as antibacterials provided that they are delivered to cells. One promising oligonucleotide is peptide nucleic acid (PNA), a synthetic DNA analog with neutral peptide-like backbone. Unfortunately, bacteria do not import short oligonucleotides, including PNA, from the environment and efficient non-invas...

[3H]Ponasterone A (PNA) of high specific activity has been used to identify and begin characterization of ecdysteroid (formerly called ecdysone) receptors in cytoplasmic and nuclear fractions of imaginal discs of Drosophila melanogaster. The equilibrium Kd of the observed macromolecular binding, 3--4 X 10(-9) M PNA, is in good agreement with the minimal concentration required for induction of c...

In an attempt to improve physico-chemical and biological properties of peptide nucleic acids (PNAs), particularly water solubility and cellular uptake, the synthesis of chimeric oligomers consisted of PNA and phosphono-PNA analogues (pPNAs) bearing the four natural nucleobases has been accomplished. To produce these chimeras, pPNA monomers of two types containing N-(2-hydroxyethyl)phosphonoglyc...

We have used time-of-flight secondary ion mass spectrometry (TOF-SIMS) to examine different immobilization processes of peptide nucleic acid (PNA) and their influence on the hybridization efficiency of unlabeled DNA fragments to complementary PNA. Two different approaches have been used to immobilize PNA onto Au surfaces. One method was to immobilize thiolated PNA in a single step reaction to t...

We have created a hybrid i-motif composed of two DNA and two peptide nucleic acid (PNA) strands from an equimolar mixture of a C-rich DNA and analogous PNA sequence. Nano-electrospray ionization mass spectrometry confirmed the formation of a tetrameric species, composed of PNA-DNA heteroduplexes. Thermal denaturation and CD experiments revealed that the structure was held together by C-H+-C bas...

Anti-miRs are oligonucleotide inhibitors complementary to miRNAs that have been used extensively as tools to gain understanding of specific miRNA functions and as potential therapeutics. We showed previously that peptide nucleic acid (PNA) anti-miRs containing a few attached Lys residues were potent miRNA inhibitors. Using miR-122 as an example, we report here the PNA sequence and attached amin...

Abbreviations: DNA: Deoxyribonucleic Acid; RNA: Ribonucleic Acid; PNA: Peptide Nucleic Acid; WC: Watson-Crick; HG: Hoongsteen; NMM: N-Methyl Morpholine; DSC: Differential Scanning Calorimetry; RP-HPLC: Reverse Phase High Performance Liquid Chromatography; DsRed: Red Fluorescent Protein; SNPs: Single Nucleotide Polymorphisms; ACE: Affinity Capillary Electrophoresis; PNA-FISH: PNA Fluorescence In...

DNA detection based on peptide nucleic acid (PNA)–DNA hybridization is emerging as an important method in the area of DNA microarrays and biosensors because PNA shows remarkable hybridization properties. In this work, we provide a novel, simple, sensitive, and selective strategy based on a PNA– graphene oxide (GO) assembled biosensor for fluorescence turn-on detection of DNA, in which the new n...