Many clinically important or mechanistically interesting inhibitors react with enzymes by a branched pathway in which inactivation of the enzyme and formation of product are competing reactions. The steady-state kinetics for this pathway [Waley (1980) Biochem. J. 185, 771-773] gave equations for progress curves that were cumbersome. A convenient linear plot is now described. The time (t1/2) for...

We show that the weak solutions of parabolic equation ∂tu−∆u+ b(t, x) · ∇u = 0 with vector field b(t, x) satisfying form-boundedness condition constitute a Feller evolution family and, thus, determine a strong Markov process. Our proof uses a Moser-type iterative procedure and an a priori estimate on the L-norm of the gradient of solution in terms of the L-norm of the gradient of initial function.

Figures Figure S1. X-ray structure packing diagram (top) and asymmetric unit (bottom) of K[DIDTC] Figure S2. X-ray structure packing diagram (top) and asymmetric unit (bottom) of K[ImDTC] Figure S3. X-ray structure packing diagram (top) and asymmetric unit (bottom) of K[PyrDTC] Figure S4. Optimized geometries of the DTC anions at a PCM/B3LYP/6-31+G(d,p) theory level. Figure S5. TD-DFT calculate...

We evaluated the performance of four frequently used cholesterol test systems, using split-sample measurements with a panel of 79 patients' specimens and isotope dilution gas chromatography-mass spectrometry (ID GS-MS) as a comparison method. The test systems were from Beckman, a Boehringer Mannheim, Merck, and Johnson & Johnson Clinical Diagnostics, performed on the Synchron CX7, Hitachi 717, ...

We measured the critical thermal maxima (CTMax) of tadpoles of Buergeria japonica inhabiting streams and geothermal hot springs. The CTMax of all B. japonica tadpoles was over 41 C, indicating that the tadpoles are heat-tolerant. At Rushan, we monitored the temporal and spatial distribution of tadpoles over a 24-h period in a 220 cm X 70 cm-plot that is fed by a geothermal hot spring. The therm...

BACKGROUND The question of whether training-induced left ventricular hypertrophy in athletes is a physiological rather than a pathophysiological phenomenon remains unresolved. The purpose of the present study was to detect any abnormalities in cardiac function in hypertrophic hearts of elite cyclists and to examine the response of myocardial high-energy phosphate metabolism to high workloads in...