BACKGROUND Transfusion of platelet concentrate is often used to treat bleeding in patients on platelet inhibitors, but little is known about its efficacy between different inhibitors. We assessed the effect of ex vivo platelet supplementation on platelet aggregability in blood samples from patients treated with acetylsalicylic acid (ASA), clopidogrel, or ticagrelor. METHODS Platelet aggregabi...

Platelet-rich plasma (PRP) has been clinically used as an easily prepared growth factor cocktail that can promote wound healing, angiogenesis, and tissue remodeling. However, the therapeutic effects of PRP are still controversial, due partly to the lack of optimized and standardized preparation protocols. We used whole blood (WB) samples to optimize the preparation protocols for PRP, white bloo...

Introduction Cartilage lesions are the most common cause of chronic knee pain. The current treatment options consist in conservative strategies, such as visco-supplementation and platelet-rich plasma injections, and surgical management including debridement of the chondral lesion, bone marrow stimulation with microfractures and osteochondral transplant. In this study we combined arthroscopic mi...

Repeated transfusions with platelets from randomly selected donors lead to HLA alloimmunization in about 50% of patients due to lymphocyte contamination of platelet concentrates. Attempts to remove the leukocytes from the platelet concentrates by additional centrifugation steps led to substantial loss of platelets. We report a new procedure for removal of almost all leukocytes with excellent pl...

negative control antibody. The CD40 ligand antibody completely abrogated the induction of PGE2. At 1/200 dilution, platelet concentrate supernatants still induced PGE2 production. High concentrations of bioactive CD40 ligand in platelet transfusions, perhaps in concert with other mediators, might explain the persistence of transfusion reactions even when white blood cells are removed before pla...