In previous equilibrium binding studies, Dictyostelium discoideum plasma membranes have been shown to bind actin and to recruit actin into filaments at the membrane surface. However, little is known about the kinetic pathway(s) through which actin assembles at these, or other, membranes. We have used actin fluorescently labeled with N-(1-pyrenyl)iodoacetamide to examine the kinetics of actin as...

Materials used for medical applications (e.g., the cellular scaffold) should have not only specific chemical composition, but surface layer properties as well. For this reason, a method which enables an increase in number of pores, wettability surface, and improvement conditions nutrient transportation into membrane is being studied. The plasma dielectric barrier discharge was applied modificat...

Treatment of tissues, isolated cells, and cell homogenates and fractions with a phosphatidylinositol-specific phospholipase C has demonstrated a selective release of several enzymes from plasma membranes. The purified phospholipase has no detectable action on mammalian phospholipids other than phosphatidylinositol and although this phospholipid appears to form only a very small proportion of th...

Both organic and inorganic solutes can be transported across biological membranes against their electrochemical gradient, by coupling to the downhill movement of another species. In animal cells, the underlying mechanism most often involves symport (cotransport) or antiport (countertransport) with either sodium or hydrogen ions. The energy invested in the uphill translocation of solutes is ulti...

The major plasma lipids, cholesteryl ester, triacylglycerol, cholesterol and phospholipid, are insoluble in water. They are transported in the circulation as lipoproteins-metabolically diverse macromolecules in which the various lipids are associated with specific polypeptides (the apoproteins). There are four main lipoprotein classes, defined by their size and by the density range within which...

Plasma membranes isolated from the fast-growing, maximal-deviation, Morris hepatoma 3924A exhibit remarkable changes in lipid composition, lipid peroxidation and to some extent in the physical state with respect to rat liver plasmalemmas. A correlation appears to exist between the lower phospholipid: protein ratio, higher cholesterol: phospholipid ratio, lower rate of lipid peroxidation and dec...

Prior studies have demonstrated the presence of specific binding sites for insulin on nuclei isolated from rat liver. In the present study to determine the subnuclear location of these insulin binding sites, intact nuclei were first incubated with ‘ZzI-insulin and then subfractionated. Approximately 70% of specific insulin binding was associated with the nuclear membrane fractions. When highly ...

We examined the subcellular localizations of NAD(P)H oxidase, a reactive oxygen species (ROS)-producing enzyme, in fetal membrane chorion laeve trophoblasts from preterm or term pregnant women with or without chorioamnionitis (CAM). Ultrastructural enzyme histochemistry for NAD(P)H oxidase was used. In fetal membranes without CAM, approximately one quarter of the chorion laeve trophoblasts (25....

phospholipase a1 is known as an effective catalyst for hydrolysis of various phospholipids in enzymatic vegetable oil degumming. immobilization is one of the most efficient strategies to improve its activity, recovery and functional properties. in this study, chitosan-co-polyethylene oxide (90:10) nanofibrous mat was successfully fabricated and modified with atmospheric plasma at different time...

SYNOPSIS. H + V-ATPases are well known energizers of endomembranes; thus they play a key role in the acidification of vacuoles and vesicles. More recently it has become clear that they energize many plasma membranes as well. In epithelial cells H + V-ATPases usually energize apical plasma membranes in the same sense that Na + /K + P-ATPases usually energize basolateral plasma membranes. Example...