This study was undertaken to isolate ligninase-producing white-rot fungi for use in the extraction of fibre from pineapple leaf agriwaste. Fifteen fungal strains were isolated from dead tree trunks and leaf litter. Ligninolytic enzymes (lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lac)), were produced by solid-state fermentation (SSF) using pineapple leaves as the substrate...

Papain was purified from spray-dried Carica papaya latex using aqueous two-phase system (ATPS). Then it was recovered from PEG phase by in situ immobilization or preparing cross-linked enzyme aggregates (CLEAs). The Plackett-Burman design and the central composite design (CCD) together with the response surface methodology (RSM) were used to optimize the APTS processes. The highly purified papa...

The aim of this study was to evaluate the growth conditions affecting production of hosphatidylinositol-specific phospholipase-C (PIPLC) by Staphylococcus aureus ATCC 9144. A simple colorimetric method for direct estimation of phospholipids was adapted for rapid assaying of PIPLC. First phase of the study involved preliminary screening of nutritional and physical parameters. In the second phase...

The interaction effect of eight operating factors on the degradation three organic dyes different structures (Cibacron green, methylene blue and methyl orange) has been studied. Effect had evaluated statistically using Plackett-Burman screening design which ex-tracted valuable information most important parameters their interactions. goodness model fit was checked by determination coefficient R...

Exo-polysaccharides like dextran produced by Weissella sp. have a wide range of applications in the food, pharmaceutical and other industries. This biopolymer and its derivatives like iron dextran, clinical dextran are rapidly emerging as a new and industrially important products. Dextran a polymer of glucose is produced using sucrose rich media with nitrogen source. It also requires efficient ...