Cacao beans must be subjected to fermentation before they are used in making chocolate, and their commercial value is related to a proper procedure. Saccharomyces rosei, Hansenula anomala, Pichia fermentans, Pichia membranaefaciens, and Trichosporon cutaneum were found in fermenting cacao beans. All species isolated during the investigation grew on cacao pulp, but only S. rosei, H. anomala, and...

Having decided on yeast as a production host, the choice of species is often the first question any researcher new to the field will ask. With over 500 known species of yeast to date, this could pose a significant challenge. However, in reality, only very few species of yeast have been employed as host organisms for the production of recombinant proteins. The two most widely used, Saccharomyces...

bioconversion of cellulosic material into bioethanol needs cellulase complex enzymesthat contain endoglucanase, exoglucanase and beta glucosidase. one of the most important organisms that produce cellulases is the filamentous fungi, trichoderma reesei which able to secrete large amounts of different cellulases. these enzymes are probably the most widely used cellulases industrially, however, th...

We study the growth of colonies of the yeast Pichia membranaefaciens on agarose film. The growth conditions are controlled in a setup where nutrients are supplied through an agarose film suspended over a solution of nutrients. As the thickness of the agarose film is varied, the morphology of the front of the colony changes. The growth of the front is modeled by coupling it to a diffusive field ...

The mutant IGC 40 x 1001 of the yeast Pichia anomala IGC 4380, which displays inverse diauxic growth in a medium with glucose and malic acid, was studied to elucidate the biochemical mechanisms underlying that behavior. Time course changes of enzyme activities during growth of the mutant in that mixture of substrates indicated that the gluconeogenic enzymes remained active during the first phas...

The recent elucidation of the enzyme systems involved in nitrate reduction in Neurospora crassa (Evans and Nason, 1952; Nason and Evans, 1953); Bacillus pumilis (Taniguchi et al., 1953); Escherichia coli (Taniguchi et al., 1956; Nicholas and Nason, 1955) and higher plants (Evans and Nason, 1953), has renewed interest in the general problem of nitrate reduction and nitrate assimilation in microo...

background identification of novel pathogenic bacteria always has a crucial role in clinical microbiology to defeat uncontrolled diseases. objectives sample preparation platform has been developed for rapid and simple detection of infectious organisms from the point of care diagnostics and molecular manipulation by using three novel genomic dna isolation protocols. materials and methods most of...