Glycerol 3-phosphate dehydrogenase 1 (GPD1) acts as a tumor suppressor in various types of cancer. However, the mechanisms GPD1 anti-tumor remain unclear breast This study aims to explore function and clinical relevance We confirmed that inhibited ability proliferation, migration, invasion overexpression cancer cells by CCK-8, wound healing, Transwell assays, respectively. found activated lipid...

Our study was aimed at exploring the roles of lncRNA RP11-400K9.4 (RP11-400K9.4) on hypoxia/reoxygenation (H/R) -induced cardiomyocytes apoptosis. H/R model constructed in rat primary (PC) and H9c2 cells. In this study, results showed that significantly induced apoptosis PC The expression upregulated H/R-induced cells, but miR-423 downregulated. Silencing could attenuate We also found a potenti...

Purpose: To investigate the role of centrosomal protein55 (CEP55) in melanoma. 
 Methods: The CEP55 expression human melanoma cells and normal melanocytes were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) western blot. Cell proliferation was evaluated colony formation CCK8 assays, while cell cycle assessed flow cytometry. Transwell assay used to migr...

Introduction Thyroid cancer is a malignant disease with poor prognosis. The PI3K/Akt/mTOR and Cyclin-Dependent Kinase 4 (CDK4) pathways are vital regulators of tumor cell proliferation and survival. Therefore the present study was designed to use dual inhibition of such pathways to kill thyroid cancer cells. Methods and materials The effects of each inhibitors on human ATC and...

(1) Background: The purpose of this study was to investigate the effects low-dose non-thermal atmospheric plasma (NTP) on proliferation and migration human immortalized keratinocytes (HaCaT cells) its molecular mechanisms. (2) Methods: NTP HaCaT cells were detected by cell viability, wound healing assay, cycle, mitochondrial membrane potential detection, western blot, role reactive oxygen speci...

The cellular kinase Akt is a key controller of cellular metabolism, growth, and proliferation. Many viruses activate Akt due to its beneficial effects on viral replication. We previously showed that wild-type (WT) simian virus 40 (SV40) large T antigen (TAg) inhibits apoptosis via the activation of PI3K/Akt signaling. Here we show that WT TAg expressed from recombinant adenoviruses in U2OS cell...

Therapies targeting MAPK and AKT/mTOR signaling are currently being evaluated in clinical trials for several tumor types. However, recent studies suggest that these therapies may be limited due to acquired cancer cell resistance and a small therapeutic index between normal and cancer cells. The identification of novel proteins that are involved in MAPK or AKT/mTOR signaling and differentially e...

This study was designed to identify the molecular mechanisms of phosphatidylinositol 3-kinase (PI3K)-induced actin filament remodeling and cell migration. Expression of active forms of PI3K, v-P3k or Myr-P3k, was sufficient to induce actin filament remodeling to lead to an increase in cell migration, as well as the activation of Akt in chicken embryo fibroblast (CEF) cells. Either the inhibitio...

BACKGROUND Deregulation of the phosphatidylinositol 3-kinases (PI3K)/Akt/mammalian target of rapamycin (mTOR) pathway plays a central role in tumor formation and progression, providing validated targets for cancer therapy. S9, a hybrid of alpha-methylene-gamma-lactone and 2-phenyl indole compound, possessed potent activity against this pathway. METHODOLOGY/PRINCIPAL FINDINGS Effects of S9 on ...

Myocardial infarction (MI) has been shown to induce endothelial dysfunction in peripheral resistance arteries and thus increase peripheral resistance. This study was designed to investigate the underlying mechanisms of post-MI-related dysfunctional dilatation of peripheral resistance arteries and, furthermore, to examine whether exercise may restore dysfunctional dilatation of peripheral resist...