The recognition in the early 1960s by Morifusa Eto that tri-o-cresyl phosphate (TOCP) is hydroxylated by the cytochrome P450 system to an intermediate that spontaneously cyclizes to a neurotoxic phosphate (saligenin phosphate ester) ignited the interest in this group of compounds. Only the ortho isomer can cyclize and clinically cause Organo Phosphate Induced Delayed Neurotoxicity (OPIDN); the ...

BACKGROUND (18)F-Fluorodeoxyglucose (FDG)-positron emission tomography (PET) imaging of atherosclerosis in the clinic is based on preferential accumulation of radioactive glucose analog in atherosclerotic plaques. FDG-PET is challenging in mouse models due to limited resolution and high cost. We aimed to quantify accumulation of nonradioactive glucose metabolite, FDG-6-phosphate, in the mouse a...

Enzyme-catalyzed phosphoryl transfer reactions have frequently been suggested to proceed through transition states that are altered from their solution counterparts, with the alterations presumably arising from interactions with active-site functional groups. In particular, the phosphate monoester hydrolysis reaction catalyzed by Escherichia coli alkaline phosphatase (AP) has been the subject o...

In recent years acyl phosphates have received much attention. For example, acetyl (1, 2), propionyl (3, 4), butyryl (5, 6), carbamyl (7, 8), and aspartyl (9) phosphates have been studied in detail. In fermentative bacteria the role of phosphotransacetylase, which facilitates an interconversion of acetyl phosphate and acetyl coenzyme A has been well documented (1,2,10-12). An enzyme capable of c...

Parathyroid hormone (PTH) increased intracellular cyclic AMP and reduces Na+/phosphate cotransport activity in OK cells [Malmström & Murer (1986) Am. J. Physiol. 251, C23-C31; Caverzasio, Rizzoli & Bonjour (1986) J. Biol. Chem. 261, 3233-3237]. It was also shown that PTH activates phosphoinositide metabolism in OK cells [Hruska, Moskowitz, Esprit, Civitelli, Westbrook & Huskey (1987) J. Clin. I...

~-Aspartate:2-oxoglutarate aminotransferase (EC 2.6.1.1) of higher organisms exists as cytoplasmic and mitochondrial isozymes with a high degree of sequence homology. This report describes an NMR study of the environment of the phosphate of the pyridoxal 5’-phosphate cofactor in the mitochondrial isozyme as revealed by the effect of pH on its 31P NMR chemical shift. Our previous study of the cy...

A study of PVD coatings, solid-in-liquid suspensions, and an ashless phosphate ester in oil at the upper die-workpiece interface when cold rotational rotary forging martensitic steel. The aim was to gain tribological insight for advanced near net-shape process development. test method a cylinder upsetting operation using industrial-scale press, lubricants were applied via in-process spraying. F...

Two synthesized eco-epoxy components based on TA: (A) glycidyl ether and (B) phosphate ester, are used, as a replacement for the Bisphenol A (BPA) epoxy component, bonding aluminum (Al) carbon fiber reinforced polymer (CFRP). Their effect mode I fracture toughness (GI) is evaluated by Double Cantilever Beam (DCB) testing while using Digital Image Correlation (DIC) in-situ crack tip monitoring. ...

this study presents the influence of hydroxypropyl-β-cyclodextrin (hpbcd) on the aqueous solubility of acyl esters of cyproterone. first, a number of esters of cyproterone were synthesized. then the phase solubility analysis of the compounds in the presence of hpbcd was investigated in phosphate buffer solution at a ph of 7.4. to gain a better understanding of the complexation mechanism, the sy...