In lactococci, transamination is the first step of the enzymatic conversion of aromatic and branched-chain amino acids to aroma compounds. In previous work we purified and biochemically characterized the major aromatic aminotransferase (AraT) of a Lactococcus lactis subsp. cremoris strain. Here we characterized the corresponding gene and evaluated the role of AraT in the biosynthesis of amino a...

Higher alcohols and esters are key compounds from fermentation that help define the character of beer. mainly produced by yeast via Ehrlich amino acid degradation pathway especially leucine, isoleucine, valine, phenylalanine. Augmenting concentration these acids can enhance output pathway. Unlike previous studies, work reported here explores possibility combining endo- exo-proteases to selectiv...

This study is aimed at screening non-Saccharomyces for amino acids decarboxylation potentials. The yeasts were isolated from banana fruit and honey purchased markets in Rivers State. isolation molecular identification of yeast isolates according to standard microbiological procedures. A plate assay method acid (biogenic amine production) was used. Wild Non-Saccharomyces (NSY) identified as Cand...

The peptide bond formation of N-protected non-coded amino acids having different structures as acyl donor substrates that is catalyzed by thermoase in organic media was investigated. In these reactions, N-protected l-non-coded amino acids, including l-Orn, l-Cit, -aminobutyric acid (l-Abu) and phenylalanine homologues, were used as the acyl donors and phenylalanine derivatives were used as the ...

A cell line of Eschscholtzia californica selected for meta-fluorotyrosine (MFT) tolerance was found to have 10-fold increased levels of phenylalanine and tyrosine compared to the parent line, while most other amino acids were only increased 2-fold. Tracer experiments with shikimic acid in the presence of MFT showed that the biosynthesis of the aromatic amino acids was not impaired in the tolera...

Variation of plasma free amino acids (PFAAs) is an essential feature of protein metabolic abnormalities in cancer patients. But there still little data about the cancer tissue free amino acid (TFAAs) profiles, including their patterns and correlations with PFAAs. To evaluate the variation in PFAAs and cancer TFAAs in patients with lung cancer, including their patterns and correlations, we inves...

The effects of glucagon on the output of amino acids by the isolated rat liver perfused with recirculating medium were to decrease that of glycine, glutamate, and phenylalanine; to increase greatly that of leucine, isoleucine, valine, and tyrosine; and to increase slightly that of lysine and histidine. In livers perfused with nonrecirculating medium containing amino acids at concentrations foun...

Shiio, Tsuru (Washington State University, Pullman), and Bruce A. McFadden. Cell-free amino acid-incorporating system from Pseudomonas indigofera. J. Bacteriol. 90:978-983. 1965.-A cell-free preparation from Pseudomonas indigofera incorporated C(14)-phenylalanine and C(14)-leucine into a product which was insoluble in hot trichloroacetic acid. The phenylalanine incorporation process, which had ...

BACKGROUND The liver plays a key role in amino acid metabolism. In former studies, a ratio between branched-chain and aromatic amino acids (Fischer's ratio) revealed associations with hepatic encephalopathy. Furthermore, low concentrations of branched-chain amino acids were linked to sarcopenia in literature. Encephalopathy and sarcopenia are known to dramatically worsen the prognosis. Aim of t...