1. Lysosome-rich fractions were obtained from foetal liver tissues as early as 35 days uterine age. Foetal lysosomes showed the same ;structure-linked latency' and acid hydrolytic potentiality characteristic of their adult counterparts. 2. The osmotic stability of lysosome-rich fraction from foetal guinea-pig liver tissue was greater than that of the corresponding adult lysosome fractions, p-ni...

Previous results of the comparison of colors given by indicators in alkaline buffers and pure aqueous sodium hydroxide have been repeated and confirmed. The electrometric determinations show that the sodium hydroxide was pure and gave theoretical values for the concentration of hydroxyl ion. The slight but distinct neutralising effect of dilute solutions of alkali has been measured electrometri...

Previous papers in this series have dealt with the fractionation and activity of ,-glucuronidase in mouse-liver suspensions ('homogenates'). Identical fractionation of the enzyme was achieved by centrifuging at high speeds or by buffering to slightly acid pH. The sedimentable fraction was associated with subcellular particles of all sizes, and these were agglutinated in acid buffers. In water s...