conclusions over-expression of the synthetic cpc/β protein in the bacterial system (escherichia coli bl-21) showed that e. coli can be used as a basis for further research to produce this desired protein in large quantities. results the sds-page analysis and dot blotting confirmed the production of recombinant c-pc/β in the bacterial expression system. over-expression of cpcb gene was optimized...

the aim of this paper is to minimize output phase noise for the pure signal synthesis in the frequency synthesizers. for this purpose, first, an exact mathematical model of phase locked loop (pll) based frequency synthesizer is described and analyzed. then, an exact closed-form formula in terms of synthesizer bandwidth and total output phase noise is extracted. based on this formula, the phase ...

Background: Acute-phase proteins (APPs) may be increased due to different stresses during estrus phase in farm animals. Aims: Determining changes of APPs at different phases of non-synchronized estrous cycle group (NSEG), and Ovsynch-synchronized estrous cycle group (SEG) in Holstein cows. Methods: Twelve non-pregnant clinically and paraclinicaly healthy Holstein cows with a body condition scor...

the purpose of this study was to evaluate the changes in the serum concentrations of haptoglobin (hp), serum amyloid a (saa) and igg, iga in calves with diarrhea caused by rotavirus, coronavirus, escherichia coli f5 and eimeria species. the experiment was carried out on 40 diarrhoeic and 10 non-diarrhoeic calves (group c). a total of 13 calves were infected with rotavirus or coronavirus (group ...

Hydrophobic interaction chromatography (HIC) is a chromatographic technique that mainly targets the separation of biomolecules (intact proteins, monoclonal antibodies, etc.) based on difference in surface hydrophobicity while applying non-denaturing conditions. This protocol paper provides guidelines for setting-up robust HIC analysis and considers instrument configuration, mobile-phase sample ...

Phase transitions of linear multivalent proteins control the reversible formation of many intracellular membraneless bodies. Specific non-covalent crosslinks involving domains/motifs lead to system-spanning networks referred to as gels. Gelation transitions can occur with or without phase separation. In gelation driven by phase separation multivalent proteins and their ligands condense into den...

Acute Respiratory Distress Syndrome (ARDS) continues to have a high mortality. Currently, there are no biomarkers that provide reliable prognostic information to guide clinical management or stratify risk among clinical trial participants. The objective of this study was to probe the bronchoalveolar lavage fluid (BALF) proteome to identify proteins that differentiate survivors from non-survivor...

Objective(s) Sickle cell disease is a genetic disorder characterized by chronic haemolytic anaemia. Haemoglobin S containing red blood cells may be susceptible to oxidative stress due to imbalance between production of reactive oxygen species and the countering effect of the various antioxidants present in the body. Materials and Methods We evaluated some antioxidant enzymes which include gl...