PREMISE OF THE STUDY The earliest eukaryotes were likely flagellates with a centriole that nucleates the centrosome, the microtubule-organizing center (MTOC) for nuclear division. The MTOC in higher fungi, which lack flagella, is the spindle pole body (SPB). Can we detect stages in centrosome evolution leading to the diversity of SPB forms observed in terrestrial fungi? Zygomycetous fungi, whic...

Background: The aim of this prospective study was to investigate the influence of long-term physical activity on HDL quality, reflected by serum amyloid A (SAA) and surfactant protein B (SPB). Methods and results: 109 healthy subjects were recruited, 98 completed the study. Participants perform within the calculated training pulse for 8 months. The performance gain was measured/quantified by bi...

In the filamentous fungus Aspergillus nidulans, cytokinesis/septation is triggered by the septation initiation network (SIN), which first appears at the spindle pole body (SPB) during mitosis. The coiled-coil protein SNAD is associated with the SPB and is required for timely septation and conidiation. We have determined that SNAD acted as a scaffold protein that is required for the localization...

We used two-photon excitation fluorescence fluctuation spectroscopy with photon counting histogram (PCH) analysis as a new tool to study the binding of globular proteins to colloidal particles in situ. Whereas fluorescence fluctuations are traditionally evaluated by calculating the autocorrelation function (fluorescence correlation spectroscopy), a complementary PCH analysis has been performed ...

The discussion of resonance mechanisms for artificial structural units has always been a key to producing highly efficient, active and tunable meta-devices in the fields controlling surface plasmon polaritons (SPPs) generate plasmonic bending beams (SPBs). In this study, an array 20 antisymmetric double V-shaped structures was designed SPB. arms were panned control electric field intensity dist...

Massive B-type pulsators such as β Cep and slowly pulsating B (SPB) stars pulsate due to layers of increased opacity caused by partial ionization. The increased opacity blocks the energy flux to the surface of the stars which causes the layers to rise and the opacity to drop. This cyclical behavior makes the star act as a heat engine and the star will thus pulsate. For β Cep and SPB stars the i...

Solitary plasmacytoma (SP), histologically indistinguishable from multiple myeloma (MM), is a kind of malignant tumor characterized by the proliferation of monoclonal plasma cells. SP is an independent subtype of plasmacytoma, including extramedullary plasmacytoma and solitary plasmacytoma of bone (SPB) [1]. Most solitary plasmacytomas progress to MM, and are usually treated in the department o...

BACKGROUND Budding yeast is a unique model for exploring differential fate in a cell dividing asymmetrically. In yeast, spindle orientation begins with the old spindle pole body (SPB) (from the preceding cell cycle) contacting the bud by its existing astral microtubules (aMTs) while the new pole delays astral microtubule organization. This appears to prime the inheritance of the old pole by the...

Yeast spindle pole bodies (SPBs) with attached nuclear microtubles were enriched approximately 600-fold from yeast cell extracts. 14 mAbs prepared against this enriched SPB fraction define at least three components of the SPB and spindle. Immunofluorescent staining of yeast cells showed that throughout the cell cycle two of the components (110 and 90 kD) were localized exclusively to the SPB re...

Centrosomes play critical roles in the cell division cycle and ciliogenesis. Sfi1 is a centrin-binding protein conserved from yeast to humans. Budding yeast Sfi1 is essential for the initiation of spindle pole body (SPB; yeast centrosome) duplication. However, the recruitment and partitioning of Sfi1 to centrosomal structures have never been fully investigated in any organism, and the presumed ...