We selected a novel biotin-binding peptide for sensing biotin, biotinylated proteins, and nucleotides. From 15-mer library displayed on the RNA coliphage Qβ, 15-amino acid long (HGHGWQIPVWPWGQG) hereby referred to as nanotag was identified selectively bind biotin. The target selection achieved through panning with elution by infection. tested transducer an immunogenic epitope of foot-and-mouth ...

Abstract Background Bacteriophage (phage) to augment antibiotic efficacy is a possible therapeutic option in the era of antimicrobial resistance. Studies date have assessed phage-antibiotic synergy (PAS), however, its may be dependent upon mechanism action. Here, we report our in-vitro evaluation antagonism (PAA) among phage and protein synthesis inhibitor combinations multidrug-resistant clini...

Aberrant glycosylation is a universal feature of cancer cells. There are quantitative and qualitative changes in expression of gangliosides observed in tumors of a neuroectodermal origin such as neuroblastoma, melanoma and astrocytoma. The presence of large amounts of GD2 ganglioside on neuroblastoma cells, as compared to normal cells, opens the possibilities to use the tumor-associated carbohy...

a random 12 mers phage library was used to screen a pool of immunoglo¬bulin fractions obtained from vitiligo patients. subsequent to panning experiments, a panel of affinity selected phage from vitiligo patients were obtained. this panel was tested using an elis a for their reactivity with pooled sera from patients and normal controls. among the 16 randomly selected clones, two of clones showed...

Objective(s): During the last years with increasing resistant bacteria to the most antibiotics bacteriophages are suggested as appropriate treatment option. To investigate lytic activity of bacteriophages there are indirect microbial procedures and direct methods. The present study to complement microbial procedures and investigate ultra-structural characteristics of infection bacterium-phage u...

background and objectives: dna ladder contains dna fragments of different length but with known size, used to determine the size of unknown dna molecules. different dna ladders are available for expected dna length. conserved sequences were selected for design of primers to generate dna fragments of known specific size. materials and methods: in this study, we describe a method by which dna lad...

In this article are reviewed the promising uses of phage display in areas such as microbial pathogens detection and vaccination. Phage is a molecular technique by which foreign proteins expressed at surface particles. Such phages thereby become vehicles for expression that not only carry within them nucleotide sequence encoding proteins, but have also capability to replicate. Recent data acquir...

Background and Objective: This study was carried out to isolate lytic bacteriophages against Pseudomonas aeruginosa and analyze their biological characteristics and antibacterial effects on other clinical isolates of bacteria. Methods: Isolating two lytic bacteriophages against two strains of P. aeruginosa was carried out using the double-layer agar method. After investigating the antibacteria...