Ammonium sulfate fractionated proteins of bitter gourd (Momordica charantia) were precipitated using jack bean extract as a source of concanavalin A. Concanavalin A-bitter gourd peroxidase retained nearly 86% original activity. This complex was entrapped into calcium alginate-pectin gel. Entrapped concanavalin A-peroxidase complex retained 51% original activity. Soluble and immobilized peroxida...

When plants are threaten by microbial attacks or treated with elicitors, alkalization of extracellular space is often induced and thus pH-dependent extracellular peroxidase-mediated oxidative burst reportedly takes place, especially at the site of microbial challenge. However, direct stimulus involved in activation of peroxidase-catalyzed oxidative burst has not been identified to date. Here, w...

A procedure for the extraction and isolation of the peroxidase in pig thyroid glands has been described. The enzyme has been purified by gel filtration and diethylaminoethyl chromatography, and its spectral properties suggest that the enzyme is a typical hemoprotein peroxidase, while gel filtration experiments have shown it to have a molecular weight of 104,000. The specific activity of the pre...

An acidic peroxidase was extracted from garlic (Allium sativum) and was partially purified threefold by ammonium sulphate precipitation, dialysis, and gel filtration chromatography using sephadex G-200. The specific activity of the enzyme increased from 4.89 U/mg after ammonium sulphate precipitation to 25.26 U/mg after gel filtration chromatography. The optimum temperature and pH of the enzyme...

GSH peroxidase has been purified approximately ZOO-fold from pig’s blood. With cumene hydroperoxide as substrate, kinetic analysis on the purified enzyme gave nonlinear Lineweaver-Burk plots for the hydroperoxide substrate. Linear Lineweaver-Burk plots were obtained for GSH and a limiting K, value of approximately 3 mu was obtained for GSH. A limited analysis of the rate data has been carried o...

Incubation of horseradish peroxidase with phenylhydrazine and H2O2 markedly depresses the catalytic activity and the intensity, but not position, of the Soret band. Approximately 11-13 mol of phenylhydrazine and 25 mol of H2O2 are required per mol of enzyme to minimize the chromophore intensity. The enzyme retains some activity after such treatment, but this activity is eliminated if the enzyme...

Although the importance of prosthetic groups to the activity of certain enzymes has been much studied, the part played by the protein portion of the molecule has received little attention. Haemoglobin, catalase and peroxidase all have the same prosthetic group, namely, protohaematin, yet the attachment of different proteins gives them distinct and characteristic properties. Thus haemoglobin is ...

Peroxidases have turned out to be potential biocatalyst for a variety of organic reactions. The research work reported in this communication was done with the objective of finding a convenient rich source of peroxidase which could be used as a biocatalyst for organic synthetic reactions. The studies made have shown that Luffa aegyptiaca (gourd) fruit juice contains peroxidase activity of the or...

Ficin is classified as a sulfhydryl protease isolated from the latex of fig trees. In most cases, a particular enzyme fits a few types of substrate and catalyzes one type of reaction. In this investigation, we found sufficient proofs for the intrinsic peroxidase-like activity of ficin and designed experiments to examine its effectiveness in a variety of scenarios. Ficin can transform peroxidase...

Horseradish peroxidase (HRP) has been broadly used and investigated for many analytical purposes; it is an enzyme that catalyzes the reduction of hydrogen peroxide in the presence of a reducing compound. The objective of this work was to develop a methodology for the spectrophotometric determination of the activity of peroxidase in vegetable extracts using a flow method with a sequential inject...