Um método novo, simples e sensível foi proposto para a determinação de mezlocilina. Baseia-se na amplificação da emissão de quimiluminescência (CL) gerada a partir da oxidação do luminol em meio alcalino pelo periodato de potássio. A otimização das variáveis experimentais e instrumentais que afetam o efeito da amplificação de CL foi realizada usando-se sistema de injeção em fluxo. Em condições ...

Human erythrocyte ghosts were chemically cross-linked with a family of bifunctional cross-linking reagents, imidoesters. Since the reagents are identical in their chemical structures, except for the number of methylene groups, they differ by that distance between the functional groups. The maximum distance is 4.9 A in dimethyl malonimidate, 6.1 A in dimethyl succinimidate, 8.6 A in dimethyl adi...

Human erythrocyte ghosts were chemically cross-linked with a family of bifunctional cross-linking reagents, imidoesters. Since the reagents are identical in their chemical structures, except for the number of methylene groups, they differ by that distance between the functional groups. The maximum distance is 4.9 A in dimethyl malonimidate, 6.1 A in dimethyl succinimidate, 8.6 A in dimethyl adi...

2-Aminoethylarsonic acid was prepared from 2-choloethylarsonic acid. The route constitutes a new procedure for making primary amines from haloalkanes; chloride was displaced by treatment with 2-aminoethanol at 70 degrees C, and the product was converted into the required primary amine by treatment with periodate.

We have examined the 3'-terminal sequence of the "small" structural ribosomal RNA ("13S") of hamster cell mitochondria, using a procedure involving [3H]isoniazide labeling of samples subjected to sequential periodate oxidation and beta-elimination. The terminus was found to be PyUAUUAOH, which is similar, but not identical, to the corresponding terminus of eukaryotic cytoplasmic 18S rRNA.

We labeled surface glycoproteins of norby polyacrylamide slab gel electrophoresis. mal human blood platelets, granulocytes, All major types of human leukocytes monocytes, T and B lymphocytes, and null showed different and characteristic surcells by the galactose oxidase-NaB3H4 face glycoprotein patterns. These patterns and periodate-NaB3H4 labeling techevidently also include common comniques. T...