Tissue engineering focuses on the repair and regeneration of tissues through the use of biodegradable scaffold systems that structurally support regions of injury while recruiting and/or stimulating cell populations to rebuild the target tissue. Within bone tissue engineering, the effects of scaffold architecture on cellular response have not been conclusively characterized in a controlled-dens...

Perfusion bioreactors regulate flow conditions in order to provide cells with oxygen, nutrients and flow-associated mechanical stimuli. Locally, these flow conditions can vary depending on the scaffold geometry, cellular confluency and amount of extra cellular matrix deposition. In this study, a novel application of the immersed boundary method was introduced in order to represent a detailed de...

B ioreactors allowing direct perfusion of culture medium through tissue-engineered constructs may overcome diffusion limitations associated with static culturing, and may provide flow-mediated mechanical stimuli. The hydrodynamic stress imposed on cells in these systems will depend not only on the culture medium flow rate but also on the scaffold three dimensional (3D) micro-architecture. We pe...

Limited available water resources have rendered wastewater reuse an important issue to specialists in most developed countries, today. The current study works on membrane filtration for treatment of industrial wastewater. By comparing the two methods of membrane bioreactor (MBR) and hybrid membrane electro bioreactor (MEBR) processes, it finds that earlier fouling in the membrane occurs in the ...

Drug hepatotoxicity testing requires in vitro hepatocyte culture to maintain the long-term and stable liver specific functions. We developed a drug testing platform based on laminar-flow immediate-overlay hepatocyte sandwich perfusion culture. The immediate-overlay sandwich (collagen-coated porous polymeric membrane as top overlay) protects the cells and integrity of the top collagen matrix fro...

Bioreactors allowing direct perfusion of culture medium through tissueengineered constructs may overcome diffusion limitations associated with static culturing, and may provide flow-mediated mechanical stimuli. The hydrodynamic stress imposed on cells in these systems will depend not only on the culture medium flow rate but also on the scaffold three-dimensional (3D) micro-architecture. We perf...

Three-dimensional tumor models accurately describe different aspects of the tumor microenvironment and are readily available for mechanistic studies of tumor biology and for drug screening. Nevertheless, these systems often overlook biomechanical stimulation, another fundamental driver of tumor progression. To address this issue, we cultured Ewing sarcoma (ES) cells on electrospun poly(ε-caprol...

An engineered three dimensional (3D) in vitro cell culture system was designed with the goal of inducing and controlling in vitro osteogenesis in a reproducible manner under conditions more similar to the in vivo bone microenvironment than traditional two-dimensional (2D) models. This bioreactor allows efficient mechanical loading and perfusion of an original cubic calcium phosphate bioceramic ...

Introduction: The repair of bone defects remains a great challenge for orthopedic surgeons. Functional tissue engineering using human bone marrow stromal cells (hBMSCs), in which the osteoconductive scaffolds play an important role, is considered a promising approach to treating these defects. We have reported that the polyurethane scaffolds can provide a favorable 3dimensional growing environm...