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The catabolism of homologous and heterologous 7S gamma globulin fragments obtained by pepsin and papain digestion was studied in rabbits, guinea pigs, and mice. The elimination from the circulation of I* labeled gamma globulin fragments was followed and the urinary excretion of the total and protein-bound I* activity determined. Evidence is presented that the molecular structure responsible for...

To monitor the structural integrity of therapeutic proteins, hydrogen-deuterium exchange mass spectrometry (HDX-MS) is increasingly utilized in the pharmaceutical industry. The successful outcome of HDX-MS analyses depends on the sample preparation conditions, which involve the rapid digestion of proteins at 0 °C and pH 2.5. Very few proteases are able to withstand such harsh conditions, with p...

The aim of this study was to investigate the digestibility of different Korean Hanwoo beef cuts using an in vitro digestion model, in vitro physicochemical upper gastrointestinal system (IPUGS). The four most commonly consumed cuts - tenderloin, sirloin, brisket and flank, and bottom round - were chosen for this study. Beef samples (75 g) were cooked and ingested into IPUGS, which was composed ...

Since 1836, when Theodor Schwann signalized the presence in gastric juice of the proteolytic ferment which he was the first to designate as pepsin, a large number of attempts have been made to isolate it in a pure form to determine its chemical nature and its ultimate physiological properties. In a large majority of these, and, especially, all those made before 1895, except that of Brticke (I),...

Since 1836, when Theodor Schwann signalized the presence in gastric juice of the proteolytic ferment which he was the first to designate as pepsin, a large number of attempts have been made to isolate it in a pure form to determine its chemical nature and its ultimate physiological properties. In a large majority of these, and, especially, all those made before 1895, except that of Brticke (I),...