A 'Heat treatment aqueous two phase system' was employed for the first time to purify serine protease from kesinai (Streblus asper) leaves. In this study, introduction of heat treatment procedure in serine protease purification was investigated. In addition, the effects of different molecular weights of polyethylene glycol (PEG 4000, 6000 and 8000) at concentrations of 8, 16 and 21% (w/w) as we...

In this work, the thermal stabilities of a series of polyethylene glycols (PEG 4000, 6000 and 10000) were investigated after compositing with different kinds of inorganic salts, such as ammonium molybdate tetrahydrate (AMT), NH4VO3, (NH4)2SO4, NH4NO3, Na2SO4, Na2MoO4. It was first observed that all the ammonium salts exerted leveling effects for the thermal stabilities of the PEGs. In other wor...

The bifunctional catalase-peroxidase KatG encoded by the katG gene of Burkholderia pseudomallei has a predicted subunit size of 81.6 kDa. It shows high sequence similarity to other catalase-peroxidases of bacterial, archaebacterial and fungal origin, including 64% identity to KatG from Mycobacterium tuberculosis and lesser sequence similarity to members of the plant peroxidase family. Crystals ...

Improving the specificity of α-cyclodextrin glucanotransferase is a significant issue in the field of α-cyclodextrin production. In this study, a constructed Y167H mutant α-cyclodextrin glucanotransferase with enhanced α-cyclodextrin specificity was successfully expressed and purified. Single crystals were grown using PEG 4000 as a precipitating agent by the hanging-drop vapour-diffusion method...

The in situ uptake from the rat jejunum of liposomes prepared fr >m various saturated phosphatidylcholines in the presence or absence of cholesteol was determined. Liposomes prepared from distearoylphosphatidylcholine. with or without cholesterol, were taken up at the fastest rate (apparent first-order uptake of intact liposomes with a half-life of about 15 min). The lipid components OE liposom...

N-utilization factor G (NusG) from Aquifex aeolicus (Aa) was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapor-diffusion technique. The drops consisted of 2.5 microl protein solution (approximately 30 mg ml(-1) in 20 mM Tris-HCl pH 8.0, 200 mM NaCl, 2 mM EDTA and 10 mM DTT) and 2.5 microl reservoir solution (0.085 M Na HEPES pH 7.5, 15% glycerol, 11% 2-pr...

The VAXstation 4000 Model 90 is the latest member of the VAXstation product line. Based on the NVAX CPU, the Model 90 was designed as a module upgrade to the VAXstation 4000 Model 60 system. The Model 90 has 2.7 times the CPU performance of the Model 60 and provides base-level, two-dimensional graphics performance of 266,000 vectors per second. It supports up to 128MB of memory, an SCSI-1 bus i...