Protein disulfide isomerases are overwhelmingly multi-modular redox catalysts able to perform the formation, reduction or isomerisation of disulfide bonds. We present here the biochemical characterization of three different poplar PDI isoforms. PDI-A is characterized by a single catalytic Trx module, the so-called a domain, whereas PDI-L1a and PDI-M display an a-b-b'-a' and a°-a-b organisation ...

BACKGROUND The Psoriasis Disability Index (PDI) is a widely used instrument to measure the impact of psoriasis on patients. There has not been psychometric evaluation of the Chinese version of PDI. The aim of this study was to evaluate its reliability and validity among Chinese patients with psoriasis. METHODS A multi-center, cross-sectional study was conducted at 9 hospitals including patien...

We prepared a solution-processed film comprising a drop-casted mixture of melamine-diacetylene and perylene bis(dicarboximide) (PDI). We show that the diacetylene monomers adopt distinct crystalline organization in the presence of the PDI residues. Importantly, the drop-casted diacetylene/PDI film exhibits ultraviolet light-induced conductivity, ascribed to effective transport of charge carrier...

Green fluorescent protein (GFP) has been widely used in several molecular and cellular biology applications, since it is remarkably stable in vitro and in vivo. Interestingly, native GFP is resistant to the most common chemical denaturants; however, a low fluorescence signal has been observed after acid-induced denaturation. Furthermore, this acid-denatured GFP has been used as substrate in stu...

1. The activities of protein disulphide-isomerase (PDI) and thioredoxin in catalysing disulphide bond isomerization in a protein substrate were compared by using the standard assay, namely the re-activation of 'scrambled' RNAase. 2. The specific activity of PDI was 25-fold greater than that of thioredoxin. 3. The greater efficiency of PDI compared with thioredoxin is considered to be due more t...

abstract   background: low back pain (lbp) is still a medical problem in 21 st century. having back pain and being disabled by it are not the same thing. it is common to come across with patients who have simple back pain but surprisingly totally disabled and vice versa. in clinical practice, it is important to have a proper evaluation of disability and making a clear distinction between pain a...

To generate a cytopathic effect, the catalytic A1 subunit of cholera toxin (CT) must be separated from the rest of the toxin. Protein disulfide isomerase (PDI) is thought to mediate CT disassembly by acting as a redox-driven chaperone that actively unfolds the CTA1 subunit. Here, we show that PDI itself unfolds upon contact with CTA1. The substrate-induced unfolding of PDI provides a novel mole...

Two novel "turn-on" fluorescent probes with perylene tetracarboxylic diimide (PDI) as the fluorophore and two different di-(2-picolyl)-amine (DPA) groups as the metal ion receptor (PDI-1 and PDI-2) were successfully synthesized with satisfactory yields. PDI-1 exhibited high selectivity toward Ni(2+) in the presence of various other metal cations including Zn(2+), Cd(2+) and Cu(2+) which were ex...

Post-streptococcal autoimmunity affects millions worldwide, targeting multiple organs including the heart, brain, and kidneys. To explore the post-streptococcal autoimmunity spectrum, we used western blot analyses, to screen 310 sera from healthy subjects with (33%) and without (67%) markers of recent streptococcal infections [anti-Streptolysin O (ASLO) or anti-DNAse B (ADB)]. A 58 KDa protein,...

Protein disulphide isomerase (PDI) is an oxidoreductase enzyme abundant in the endoplasmic reticulum (ER). In plants, PDIs have been shown to assist the folding and deposition of seed storage proteins during the biogenesis of protein bodies in the endosperm. Cloning and characterization of the complete set of genes encoding PDI and PDI like proteins in bread wheat (Triticum aestivum cv. Chinese...