A study was performed to investigate the genomic variations in the shrimp farm isolates of Vibrio alginolyticus and V. harveyi when the isolates were subjected to environmental stress. Samples of shrimps, water and sediment were collected from Southern Indian coastal shrimp farms. Vibrio isolates were biochemically identified and confirmed using 16S rDNA and gyrB gene specific PCR. The bacteria...

The diversity of Bacillus species isolated from the fermented soup condiment okpehe in Nigeria was studied using a combination of phenotypic and genotypic methods. Fifty strains presumptively characterized as Bacillus spp. using the API 50 CHB test were further identified by PCR of randomly amplified polymorphic DNA (RAPD) and by amplified ribosomal DNA restriction analysis (ARDRA) genotyping m...

BACKGROUND & OBJECTIVE Nosocomial infections caused by Acinetobacter spp. are a significant problem worldwide. Information on epidemiological investigation of outbreaks caused by Acinetobacter species in India is lacking. The present investigation was carried out to elucidate molecular epidemiology of Acinetobacter species isolated from nosocomial infections in a tertiary care hospital in south...

List of original papers This thesis is based on the following articles, which in the text will be referred to by their Roman numerals. Additionally, some unpublished results are presented. fingerprinting as a tool to study the genetic diversity of Rhizobium galegae. diversity of rhizobia isolated from Astragalus adsurgens growing in different geographical regions of China. Molecular evolution o...

Introduction Many different DNA-based molecular techniques are being developed for fingerprinting and to estimate genetic diversity in eukaryotic organisms. Because of ¿le relative ease in its utilization, PCR is increasingly being used as a tool in marker development. Random amplified polymorphic DNA (RAPD) markers are easier to analyze than RFLPs and are being used in breeding programs to tag...