We describe an augmented reality (AR) marker that has both a virtual appearance and physical features. We integrate a blimp with an AR marker to overcome two problems found in conventional AR applications. One problem is mobility. While a conventional AR marker is generally paper-based and static, “Aero-Marker” floats in the air and moves toward the user. Thus, the relationship between the user...

The completeness of reference libraries is often a limiting factor in the effectiveness biomonitoring using molecular tools. fact that these are built upon Sanger sequencing can create substantial bias due to poor primer fits and unoptimized lab protocols. Some taxa marine macroinvertebrates known be notoriously difficult sequence traditional, PCR-based means, only about 15% bioindicator specie...

Abstract. As an excellent local sheep breed in China, Hu have the characteristics of producing more lambs and good motherhood. The purpose this study was to identify polymorphism mitogen-activated protein kinase 5 (MAP3K5) gene determine whether it associated with body size traits (body height, length, chest circumference, cannon circumference) sheep. MAP3K5 identified by using PCR amplificatio...

forty monoconidial isolates of magnaporthe grisea were examined, for an identification of vegetative compatibility group and a characterization of genetic diversity, using rep-pcr genomic fingerprinting. the isolates were collected from weeds digitaria sanguinalis (crabgrass), setaria italica (foxtail millet), echinochloa crus-galli (barnyard millet), and some other unknown ones during 2003 - 2...

Each of the adaptamer-directed genome manipulation methods is predicated on the fact that recombination between two DNAs is enhanced by increasing the length of homology. Many of the current PCR-based genome manipulation techniques rely on very short homologies to promote recombination. In these cases homology length is dictated by the technical limits of oligonucleotide synthesis. Adaptamers c...

Endogenous non-coding small RNAs usually consist of 20–24 nucleotides, are found ubiquitously throughout the genome and play important regulatory roles in most eucaryotes. In this study, a new kind of PCR-based marker approach using multi-mapped small RNA sequences, called inter small RNA polymorphism (iSNAP), was developed. iSNAP primers were designed based on multi-mapped small RNA and its tw...

BACKGROUND Few studies have addressed the timing of cervical cytologic abnormalities and human papillomavirus (HPV) positivity during the course of an infection. It remains largely unknown how infections detected by HPV and cytology wax and wane relative to each other. The aim of this analysis was to assess the longitudinal relationship of abnormal cytology and HPV positivity in a 7-year prospe...

Wheat bread-making quality is mainly determined by glutenin proteins in the grain, which exist in a wide range of variable alleles with differential influence on processing attributes. A recently identified allele, Bx7 over-expression (Bx7(oe) ), has been showing highly significant positive effects on wheat dough strength over the normally expressed Bx7 allele. SDS-PAGE and normal RP-HPLC proce...