This paper describes the first prototype of a “Programming by demonstration” (PbD) system for position teaching of a robot manipulator. A new approach for enabling PbD using supervised learning is presented, by connecting a wearable input device for sensing human arm movements to the software controller of a robot arm. The method does not require analytical modelling of either the human arm or ...

It is now generally accepted that several types of “risks” can be recognized for built facilities and environments: they are not limited to collapses and heavy damages but involve comfort and way of life (cf. e.g. Augusti et al., 2003). The most rational way of tackling such risks and their reduction is Performance-based Design PBD (or, better, Performance-based Engineering). This lecture will ...

Polo-like kinase (Plk1) is crucial for cell cycle progression through mitosis. Here we present the molecular and structural mechanisms that regulate the substrate recognition of Plk1 and influence its centrosomal localization and activity. Our work shows that Plk1 localization is controlled not only by the polo box domain (PBD); remarkably, the kinase domain is also involved in Plk1 targeting m...

Potassium butyl dithiophosphate (PBD) was developed and introduced as a new chelating agent for heavy metal removal. The synthesized PBD were characterized by IR and NMR. The effects of pH, chelating agent dosage, and other heavy metal ions on the performance of PBD in Cd2+ removal from water are investigated. Experimental results showed that the chelating agent could be used to treat acidic he...

Polo-like kinase-1 (Plk1) phosphorylates a number of mitotic substrates, but the diversity of Plk1-dependent processes suggests the existence of additional targets. Plk1 contains a specialized phosphoserine-threonine binding domain, the Polo-box domain (PBD), postulated to target the kinase to its substrates. Using the specialized PBD of Plk1 as an affinity capture agent, we performed a screen ...

The polo-box domain (PBD) has critical roles in the mitotic functions of polo-like kinase 1 (PLK1). The replacement with partial ligand alternative through computational enrichment (REPLACE) strategy to develop inhibitors of protein-protein interactions has identified alternatives for the N-terminal tripeptide of a Cdc25C substrate. In addition, a peptide structure-activity relationship describ...

microstructural investigation of low molecular weight 1,4-polybutadiene (1,4-pbd) was studied by 1h and 13c nuclear magnetic resonance spectroscopy (nmr). the isomer contents of 1,4-cis, 1,4-trans and 1,2- vinyl in polybutadiene microstructure were determined. the number average molecular weight for low molecular weight polybutadiene was measured by nmr techniques and comparison was made with g...

The pyrrolobenzodiazepines (PBDs) are a family of covalent-binding DNA-interactive minor-groove binding agents with a thermodynamic preference for binding to 5'-Pu-G-Pu-3' sequences (Pu = Purine) but a kinetic preference for 5'-Py-G-Py-3' (Py = Pyrimidine). Using HPLC/MS methodology and a range of designed hairpin-forming oligonucleotides, the kinetics of reaction of a C8-bis-pyrrole pyrroloben...

Thiosuccinimide-linked antibody-drug conjugates (ADCs) are susceptible to drug loss over time due to a retro-Michael reaction, which can be prevented by selecting stable conjugation positions or hydrolysis of the thiosuccinimide. Here, we investigate pyrrolobenzodiazepine (PBD) ADC drug-linkers equipped with N-phenyl maleimide functionality for stable thiol conjugation via thiosuccinimide hydro...

BACKGROUND Conventional phantom-based planar dosimetry (2D-PBD) quality assurance (QA) using gamma pass rate (GP (%)) is inadequate to reflect clinically relevant dose error in intensity-modulated radiation therapy (IMRT), owing to a lack of information regarding patient anatomy and volumetric dose distribution. This study aimed to evaluate the dose distribution accuracy of IMRT delivery for na...