Pasteurella multocida strain P1059 is a highly virulent bacterium which causes fowl cholera in turkeys and chickens. A genomic library of P. multocida P1059 DNA was constructed using pUC19, expressed in Escherichia coli DH5 alpha, and screened with chicken antisera generated against P. multocida P1059. Twelve out of the 4100 clones screened were immunoreactive. Plasmids isolated from these twel...

this study evaluated the nasopharyngeal microbial flora and antimicrobial susceptibility patterns of the one hundred and thirty holstein calves with dairy calf pneumonia from dairy farms of mashhad suburb between september 2002 and august 2003 . the most common micro-organisms isolated were pasteurella multocida 80 (61.54%), mannheimia haemolytica 41 (31.54%), bacillus sp. 15 (11.54%), staphylo...

The antimicrobial susceptibilities of 421 Pasteurella haemolytica and 158 P. multocida isolates recovered from cattle with respiratory disease were determined with a microdilution minimal inhibitory concentration test system. Isolates were analyzed for patterns of resistance to ampicillin, ceftiofur, erythromycin, gentamicin, penicillin, spectinomycin, sulfachlorpyridazine, sulfadimethoxine, te...

A selective medium (CGT medium), containing clindamycin, gentamicin, potassium tellurite and amphotericin B in 5% horse-blood agar, allowed unimpaired growth of almost all strains of Pasteurella multocida, and P pneumotropica, while inhibiting other bacteria that might be encountered in upper respiratory tract secretions. With its use, P multocida was readily detected in oral swabs from four of...

Active and passive protection studies in mice using sheep antisera revealed that the immunological relationship among Pasteurella multocida Type A strains could not be correlated with their serological relationship as determined by a haemagglutination or an agglutination test. Furthermore, strains possessing similar phenol extractable antigens or heat stable antigens did not provide complete cr...

this study has been carried out with the objective of isolation and identification of agent(s) of pasteurella pneumonia in sheep and goat in iran using bacteriological and biochemical assays to be identified in the pursuant researches to be used in pasteurellosis vaccine production. to accomplish this objective, samples were gathered from areas suspicious to pasteurellosis infection and industr...

Pasteurellosis is a zoonosis often caused by cat or dog bites or scratches, or by direct exposure to their secretions. Pasteurella multocida is the main pathogen involved in infections through domestic animal bites; generally a local infection characterized by its particular virulence with consequent rapid onset. Serious infection has also been reported in persons affected by comorbidity withou...

Sublines homozygous for each of four MHC haplotypes were developed from randombred control populations of turkeys and challenged with Pasteurella multocida (capsular serogroup a, somatic serotype 3, 4) at 6 wk of age or Newcastle disease virus (NDV; Texas GB strain) at 4 wk of age. In addition, individuals from a randombred control line (RBC2) and a subline (F) of RBC2 long-term selected for in...

Fowl cholera continues to be of concern to the poultry industry, especially for turkey growers. This disease costs the turkey industry millions of dollars annually. In order to develop improved live attenuated vaccines or subunit vaccines, the outer-membrane proteins of Pasteurella multocida were examined with the use of proteomics. Of the 11 proteins total present in an outer-membrane subfract...

Forty-eight Pasteurella multocida cultures collected from rabbits over a 56-year period were examined to determine their physiological characteristics and to determine their serological types in the gel diffusion precipitin test. Generally, the physiological characteristics from 30 tests were typical for P. multocida. There were a few atypical variations in the fermentation of lactose and malto...