In this study, we optimized the fermentation medium for spore production of Paenibacillus polymyxa IN937a. Seven factors including molasses, glucose, magnesium sulfate, potassium pyrophosphate, yeast extract, zinc and ammonium sulfate are selected as basis screening affecting P. IN937a by Plackett-Burman experiment. Based on analysis matrix, result showed that were three main impact (P < 0.0...